大黄虫丸拆方对动脉粥样硬化家兔血管平滑肌细胞增殖与凋亡的影响  

Effect of the Disassembled Recipes of Dahuang Zhechong Pill on Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Atherosclerotic Rabbits

作　　者：姬媛媛[1] 刘俊田[1] 李静莉[1] 王志东[2] 刘传镐[1] 丁存晶[1] 李天霞[1] 

机构地区：[1]西安交通大学医学院药理学系,西安710061 [2]西安交通大学医学院第二附属医院普外科

出　　处：《中国中西医结合杂志》2006年第10期913-917,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题(No.30572347)

摘　　要：目的研究大黄虫丸拆方对动脉粥样硬化(AS)模型家兔胸主动脉血管平滑肌细胞(VSMC)增殖与凋亡的影响。方法采用免疫性内皮损伤合并高脂饲料喂养的方法建立家兔AS模型,通过SP免疫组化法检测增殖细胞核抗原(PCNA)在VSMC中的表达,缺口末端标记法(TUNEL)观察VSMC的凋亡情况。结果与模型对照组比较,大黄虫丸拆方各功效组分均可使AS模型家兔胸主动脉VSMC中PCNA阳性细胞显著减少(P<0.01),而凋亡阳性细胞数显著增加(P<0.01)。结论大黄虫丸各功效组分通过抑制VSMC增殖、诱导其凋亡,从而调节VSMC增殖和凋亡之间的平衡,发挥抗AS作用,其中拆方1号是主要组分。Objective To study the effects of the disassembled recipes of Dahuang Zhechong Pill （DRDZP） on proliferation and apoptosis of vascular smooth muscle cell （VSMC） of thoracic aorta in atherosclerotic rabbits, Methods The atherosclerotic rabbit model was induced by high-cholesterol diet and immune injury of endothelium, Expression of proliferating cell nuclear antigen （PCNA） in VSMC was detected by SP immunohistochemical technique, and VSMC apoptosis was observed with TUNEL technique. Results Compared with the model control, PCNA expression in VSMC decreased, while apoptotic cells increased significantly in all the groups treated by DR-DZP （P 〈 0.01 ）. Conclusion All the DR-DZP could inhibit VSMC proliferation and promote its apoptosis to modulate the balance between cell proliferation and apoptosis, so as to exert antiatherosclerotic action, among which the disassembled-recipe I is the main composition to contribute to antiatherosclerotic action of Dahuang Zhechong Pill.

关 键 词：动脉粥样硬化 血管平滑肌细胞 增殖 凋亡 大黄[庶虫]虫丸 

分 类 号：R285[医药卫生—中药学]