层粘蛋白促进复方壳多糖组织工程皮肤基底膜构建的实验研究  

Experimental study on laminins promoting basement membrane formation in composite chitosan tissue-engineered skin in vitro

作　　者：鲁元刚[1] 伍津津[1] 张教扬[1] 雷霞[1] 杨涛[1] 朱堂友[1] 杨桂红[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所皮肤科,重庆400042

出　　处：《第三军医大学学报》2006年第23期2299-2301,共3页Journal of Third Military Medical University

基　　金：国家"863"高科技术研究发展计划(2003AA205022);重庆市科技攻关计划项目(20067897)~~

摘　　要：目的证实外源性层粘蛋白能否促进复方壳多糖组织工程皮肤基底膜的构建。方法在体外培养组织工程皮肤的培养基中加入浓度为0、1、5、20μg/ml的外源性层粘蛋白,培养15d,通过HE染色及PAS染色,观察获得的组织工程皮肤的组织学特性;并利用免疫组化染色对基底膜的重要成分包括Ⅳ型胶原和层粘蛋白进行观察,观察基底膜构建情况。结果加入20μg/ml的层粘蛋白,可明显促进基底膜的形成,且免疫组化染色结果显示Ⅳ型胶原和层粘蛋白反应强阳性。结论层粘蛋白可促进复方壳多糖组织工程皮肤基底膜的构建,并呈浓度依赖性。Objective To prove the possibility of exogenous laminins to facilitate the basement membrane （BM） formation in tissue-engineered skin. Methods Laminins at the concentrations of 0, 1,5 and 20 μg/ml respectively was added into the keratinocyte growth medium to prepare composite chitosan tissue-engineered skin. After 15 d, the histological manifestation of the skin was observed by HE and PAS staining, while cytokeratin 14 （CK14）, collagen Ⅳ （ COL-Ⅳ ） and laminins（LN） at the dermal-epidermal junction were detected with immunohistochemical assays. The formation of basement membrane was assessed. Results The BM in the medium with 20μg/ml laminins developed significantly better than other groups. Immunohistochemical studies showed strong COL-Ⅳ and LN at the dermal-epidermal junction in the tissue-engineered skin. Conclusion Laminins may facilitate the formation of basement membrane in composite chitosan tissue-engineered skin in a concentration-dependent fashion.

关 键 词：组织工程皮肤 基底膜 层粘蛋白 

分 类 号：R318.08[医药卫生—生物医学工程] R322.99[医药卫生—基础医学]