多重PCR检测鲍曼不动杆菌氨基糖苷乙酰转移酶相关基因  

Detection of aminoglycoside acetyltransferases in Acinetobacter baumannii by multiplex PCR

作　　者：刘丁[1] 陈萍[1] 李玉红[1] 杨程[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所检验科感染监控室

出　　处：《第三军医大学学报》2006年第23期2320-2322,共3页Journal of Third Military Medical University

基　　金：全军"十五"医药卫生基金资助项目(01Q-096)~~

摘　　要：目的分析aac耐药基因亚型在临床分离的耐氨基糖苷类鲍曼不动杆菌中的检出情况,建立检测鲍曼不动杆菌耐药性的分子生物学方法。方法对从临床分离的42株鲍曼不动杆菌,进行药敏试验同时行aac耐药基因PCR检测。结果本地区所分离的鲍曼不动杆菌对庆大霉素耐药率最高(57%),对丁胺卡那耐药率较低(29%)。在对妥布霉素、庆大霉素、丁胺卡那至少一种耐药的鲍曼不动杆菌中,除2株细菌外,均检测到乙酰转移酶基因,其中6株有aac(6′)-Ih、20株为aac(3)-Ia、14株含aac(6′)-Ib、14株同时含aac(3)-Ia和aac(6′)-Ib。结论本地区鲍曼不动杆菌对临床常用氨基糖苷类抗生素庆大霉素耐药率高,其耐药主要是由aac(3)-Ia、aac(6′)-Ib编码的酶引起。Objective To analyze the distribution of aminoglycoside acetyltransferases （aac） gene in Acinetobacter baumannii, and to establish a molecular biology method to detect the drug-resistance of the bacteria. Methods The aac genes of 42 isolated Acinetobacter baumannii were analyzed by drug susceptibility and multiplex PCR assay. Results The isolated bacteria were more resistant to Gentamicin （57%） and less resistant to Amikacin （29%）. As to the bacteria resistant to at least one of the following aminoglycosides, Gentamicin, Tobramycin, Amikacin, aminoglycoside resistant genes were detected in all of pathogens except 2 special. Among them 6 strains with aac （6＇）-Ih, 20 strains with aac （ 3 ）-Ia, 14 strains with aac （6＇）-Ib and 14 strains were found to have both aac（6＇）-Ib and aac（3）-Ia. Conclusion Acinetobacter baumannii isolated in this district are easily resistant to Gentamicin, and it is most caused by the enzyme encoded by aac（3 ）-Ia and aac（6＇）-Ib.

关 键 词：鲍曼不动杆菌 多聚酶链反应 氨基糖苷类耐药 

分 类 号：R378.99[医药卫生—病原生物学] R345.5[医药卫生—基础医学]