用微载体培养Vero细胞制备狂犬疫苗  

Preparation of Rabies vaccine in Vero cells grown on microcarriers

作　　者：李平忠[1] 沈伟[1] 余芬[1] 温嘉纳[1] 黄成[1] 孙明波[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所生物制品四室,昆明650118

出　　处：《第三军医大学学报》2006年第23期2374-2376,共3页Journal of Third Military Medical University

摘　　要：目的研究微载体培养Vero细胞生产狂犬疫苗。方法搅拌式生物反应器培养Vero细胞,待细胞在微载体上长成单层后,用狂犬病毒aG株感染细胞。培养5d后,开始灌流收获病毒液,每天取样测定病毒滴度。经灭活后制备成试验疫苗,进行效力试验。结果3次培养,细胞密度均达到1×106/ml以上,病毒滴度都在106LD50以上,3批试验疫苗的效价分别达到5.88、6.49和5.98IU/ml。结论在生物反应罐中用微载体培养Vero细胞制备狂犬疫苗工艺合理,所获免疫原性良好,适用于工业化生产,有较好的应用前景。Objective To develop a novel process for production of rabies vaccine in Vero cells grown on microcarriers in bioreactor. Methods Vero cells were grown on the Cytodex-1 microcarriers in a 7-liter bioreactor and infected with rabies virus strain aG when the confluent monolay was formed. After an initial 5-day culture, perfusion was started and virus suspensions were harvested each day. The viral titers of rabies virus in the harvests were assayed. After inactivation with β-propiolactone, three separate trial vaccine lots were prepared and subjected to potency assay. Results During the procedure for production of the three vaccine lots, the cells density achieved above 1 × 10^6 cells/ml and the viral titers achieved above 106LD50/ml. The potency value for the three vaccine lots reached 5.88, 6.49 and 5.98 IU/ml respectively. Conclusion The process for production of Rabies vaccine in Vero cells grown on microcarriers in a bioreactor is a novel, efficient and practical way to produce viral antigen for vaccine purpose.

关 键 词：生物反应器 细胞培养 病毒培养 狂犬疫苗 

分 类 号：R512.990.3[医药卫生—内科学]