转化生长因子-β1和胰岛素样生长因子-1对人髓核细胞体外增殖活性的影响  

Effects of transforming growth factor-β1 and insulin-like growth factor-1 on proliferation of human nucleus pulposus cells in vitro

作　　者：张荣峰[1] 阮狄克[1] 张超[1] 辛洪奎[1] 

机构地区：[1]海军总医院骨科,北京市100037

出　　处：《中国脊柱脊髓杂志》2006年第11期856-860,I0006,共6页Chinese Journal of Spine and Spinal Cord

基　　金：国家自然科学基金资助项目(项目编号:30370389)

摘　　要：目的:研究转化生长因子-β1(TGF-β1)和胰岛素样生长因子-1(IGF-1)单独及联合应用对人髓核细胞体外增殖活性的影响,并观察其量效和时效关系。方法:体外分离培养人髓核细胞,将传2代细胞种于96孔板,采用噻唑蓝(MTT)比色法,观察TGF-β1和IGF-1在1%和10%血清浓度下对人髓核细胞体外增殖的调节作用及其剂量、时间与作用效果的关系。结果:在1%血清条件下,IGF-1的作用不显著,TGF-β1具有促增殖作用。在10%血清条件下,TGF-β1和IGF-1均能提高细胞的增殖活性,并且在有效浓度范围内呈剂量效应关系,TGF-β1的作用强于IGF-1。二者联合应用效果更显著。结论:TGF-β1和IGF-1均能不同程度地促进人髓核细胞的体外增殖,其效应在一定范围内与剂量和时间呈正相关,联合应用促进增殖作用更显著。Objective：To investigate the dose-dependent and time-dependent effect of transforming growth factor β1 （TGF-β1） and insulin-like growth factor-1（IGF-1） on proliferation of nucleus pulposus（NP） cells in vitro.Method：Tissue samples of NP were obtained from anterior surgical procedures performed on five donors with idiopathic scoliosis.Cells were isolated and cultured in FI2 medium supplemented with 10% fetal bovine serum （FBS）.Cells were seeded in 96-well plates at 1 ×10^3 cells/well.After synchronization,medium was replaced by F12 containing 1% or 10% FBS with either single or combination of TGF-β1 and IGF-1.Dose- dependent and time-dependent effect were examined by MTF assay.Result-In the presence of 1% FBS,the role of IGF-1 was less striking,whereas TGF-β1 had a remarkably stimulating effect on cell proliferation.In 10% FBS,both of the two growth factors had statistically significant mitogenic effects,especially for TGF-β1. The dose-dependent effect of TGF-1 and IGF-1 on cell proliferation was found within different concentrations of each growth factor （TGF-β1 1-10μg/L,IGF-1 10-100μg/L）.The time-dependent effect showed a significant elevation three days later.A synergetic effect was observed when they were used in combination.Conclusion： TGF-μ1 and IGF-1 were efficient to stimulate cell proliferation of human NP cells in vitro in a dose-dependent and time-dependent manner.The synergetic effect is achieved when they are used in combination.

关 键 词：转化生长因子 胰岛素样生长因子 髓核细胞 增殖 

分 类 号：R681.5[医药卫生—骨科学] R329.2[医药卫生—外科学]