膝骨关节炎滑膜细胞体外培养及生物学特性观察  

Biologic characteristics of human synoviocytes of gonarthritis in vitro

作　　者：张鹏[1] 石关桐[2] 郑昱新[2] 

机构地区：[1]上海市第六人民医院针推伤科,上海200233 [2]上海中医药大学附属曙光医院骨伤科

出　　处：《中国骨伤》2006年第11期656-658,共3页China Journal of Orthopaedics and Traumatology

基　　金：国家自然科学基金资助项目(30472222)

摘　　要：目的观察原发性膝骨关节炎患者滑膜细胞的体外生长特性,探讨骨性关节炎的发病机制。方法组织块培养法分离早期与晚期(依据Kellgren和Lawrence的放射学诊断标准划分)OA患者滑膜细胞进行培养,用光镜、电镜、组织化学方法等观察鉴定该细胞类型。结果患者滑膜细胞符合巨噬样滑膜细胞(A型细胞)及成纤维细胞样滑膜细胞(B型细胞)的特征,早期与晚期滑膜细胞在生长周期及细胞超微结构等方面存在差异。晚期较早期滑膜细胞生长周期长、生长极向性差、细胞内溶酶体丰富。结论组织块培养法可以有效分离人类关节滑膜细胞,建立原发性膝骨关节炎患者滑膜细胞系。Objective：To investigate the biological characteristics of human synoviocytes of primary gonarthritis in vitro. Methods： Human synoviocytes were isolated from synovial membrane（ earlier stage and later stage of OA） by tissue culture isolation, which were identified by light microscope, electro microscope, histo-chemical method. Results：The isolated cells were in acordance with the characteristics of macrophage-like synoviocytes（type-A synoviocytes） and fibroblast-like synoviocytes （type-B synoviocytes）. Differences showed in the growth cycles, differentiations and ultra microstructures between the two stages. Conclusion：Tissue culture isolation is an effective isolation of human synoviocytes to establish the human synoviocyte line of primary gonarthritis.

关 键 词：滑膜 细胞 培养的 骨关节炎 膝 

分 类 号：R459.5[医药卫生—治疗学]