咪喹莫特纳米结构脂质载体的可控制备及表征  

Controllable Preparation and Characterization of Imiquimod-loaded Nanostructured Lipid Carriers

作　　者：周华锋[1] 马全红[1] 丁艺[2] 夏强[1] 旷英姿[2] 郝晓帧[2] 顾宁[1] 

机构地区：[1]东南大学生物电子学国家重点实验室江苏省生物材料与器件高技术重点实验室 [2]东南大学化学化工系,江苏南京210096

出　　处：《过程工程学报》2006年第4期598-602,共5页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:60171005;60371027);江苏省计划委员会资助项目(编号:7607038009)

摘　　要：采用高压均质法制备咪喹莫特纳米结构脂质载体(IMQD-NLC),分别考察表面活性剂、高压均质操作参数及药物加入量对IMQD-NLC制备的影响,并对其形貌、物相、表面Zeta电位及体外释放特性等进行了表征.结果表明,选取S-40和Span-20复配作为表面活性剂,随着S-40含量的增大,IMQD-NLC的平均粒径和Zeta电位的绝对值逐渐减小,而PI值呈现增大趋势;高的高压均质循环次数制备的IMQD-NLC平均粒径和PI值均比较低;药量增大,粒子平均粒径和PI值增大,而Zeta电位并没有发生明显变化;平均包封率为88.3%;IMQD-NLC呈无定型状态;IMQD-NLC在pH1的释放介质中呈现先突释后缓释,而在pH5.5的释放介质中呈现缓慢释放.Imiquimod-loaded nanostuctured lipid carriers （IMQD-NLC） were prepared by high pressure homogenization method. The influences of surfactant, high pressure homogenization parameter and capacity loading on the preparation of IMQD-NLC were explored. Additionally, its morphology, crystalline phase, Zeta potential and release in vitro were charaeterized. The results show that there was a trend toward increasing the particle size of IMQD-NLC and decreasing the absolute value of Zeta potential with increasing the content of S-40 in the combined surfactant of S-40 and Span-20. Both the particle size and PI value were lower with the many cycle times, while ascending with increasing the content loading. IMQD-NLC, characterized by XRD, was amorphous. It was confirmed that burst release occurred followed by a sustaining release at pH 1 and a sustaining release at pH 5.5 in vitro for IMQD-NLC. Consequently the preparation of IMQD-NLC can be controlled by altering the conditions of preparation to realize sustained release effect.

关 键 词：咪喹莫特 纳米结构脂质载体 缓释 

分 类 号：R943.4[医药卫生—药剂学]