重组人粒细胞—巨噬细胞集落刺激因子工程菌诱导条件的优化研究  

Study on the Optimizing Inducement Condition of Engineering Bacteria of GM-CSF

作　　者：余琼[1] 孙怡宁[1] 王超[1] 邹积宏[1] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《黑龙江医药》2006年第6期448-450,共3页Heilongjiang Medicine journal

摘　　要：目的：本实验用粒细胞巨噬细胞集落刺激因子(GM-CSF)进行培养,探讨目的蛋白最高表达量的诱导条件。方法：通过实验条件的优化确定了适合GM-CSF表达的诱导温度、诱导时间和培养基等。结论：带有GM-CSF的基因工程菌在30℃生长最合适,培养5小时后42℃升温诱导5小时目的蛋白表达量最高。Objective:The engineering bacteria of GM-CSF were cultured in order to study on the inducement con- clifton of the highest quality of C,M-CSF.Methods:The experiment condition was optimized to determine the suitable in- ducement temperature,time and media of CM-CSF,etc.Results:Experiment demonstrates that E.coli with GM-CSF recombinant gene grows best at 30℃,After cultivating for 5 hours and raising to 42℃,inducing for 5 hours,the target protein is the largest.

关 键 词：粒细胞-巨噬细胞集落刺激因子 目的蛋白 诱导 

分 类 号：R392-33[医药卫生—免疫学]