三尖杉酯碱诱导HL-60细胞程序性死亡过程中的细胞质和细胞核出泡与染色质凝集  

APOPTOSIS OF HL-60 CELLS INDUCED BY HARRING- TONINE. MEMBRANE BLEBS, NUCLEUS BLEBS AND CHROMATIN CONDENSATION

作　　者：方敏[1] 张鸿卿[1] 薛绍白[1] 

机构地区：[1]北京师范大学生物系,北京100875

出　　处：《实验生物学报》1996年第3期221-223,共3页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金;国家教委博士点基金;国家教委开放实验室基金

摘　　要：本文利用视频显微影像反差增强技术(VideoEnhancement Contrast,VEC)对三尖杉酯碱诱导的单个HL-60活细胞程序死亡(Apo-ptosis,Apo)全过程进行了观察,结果表明每个Apo细胞在染色质凝集前都要发生细胞核的出泡,而每一个核出泡又都是由相应的质出泡所诱导的,但并不是每个质出泡都能诱导核出泡,质出泡的次数远远高于核出泡,提示核、质出泡可能与染色质凝集有关,并且核、质出泡是程序死亡细胞形成Apo小体所必需的。进一步研究则说明核、质出泡与微丝解聚和重组有关。核、质出泡虽可加速细胞程序死亡过程中的染色质凝集,但并不是程序死亡细胞染色质凝集所必需的,提示HL-60细胞程序死亡过程中的核变化和质变化可能是相对独立的。Using Video Enhancement Contrast (VEC) microscopy, we recorded the morphological changes of same HL-60 cell in the processes of apoptosis induced by har-ringtonine. Our results show that all of apoptotic cells need several nucleus blebs before their chromatin condensation. Every nucleus bleb is induced by a relative membrane bleb. The number of membrane blebs is much higher than that of nucleus blebs, so there are only some of membrane blebs which can induce nucleus blebs. It suggested that membrane and nucleus blebs probably are related to apoptotic chromatincondensation.After HL-60 cells pretreated with cy-tochalasin B(CB), apoptotic chromatin condensation delayed eight hours, but no membrane bleb, nucleus bleb and apoptotic body formed eventually. So membrane and nucleus blebs during apoptosis are related to microfilament re-organization and can accelerate apoptotic chromatin condensation, but are unnecessary for apoptotic chroma-tin condensation. All this suggested that nuclear changes and cytoplasmic changes during HL-60 cell apoptosis are independent.

关 键 词：细胞死亡 程序性 核出泡 质出泡 染色质凝集 

分 类 号：Q251[生物学—细胞生物学]