NA和5－HT对小脑脑片浦肯野细胞自发及诱发电活动的影响  

EFFECTS OF NA AND 5-HT ON THE SPONTANEOUS AND EVOKED ACTIVITIES OF PURKINJE CELLS IN CEREBELLAR SLICE

作　　者：胡卫红[1] 王建军[1] 张敏跃[1] 余启祥 

机构地区：[1]南京大学生物科学与技术系生理教研室,中国药科大学中药药理教研室

出　　处：《生理学报》1996年第6期581-586,共6页Acta Physiologica Sinica

基　　金：国家自然科学基金;国家教委年轻教师基金;国家教委留学回国人员科研资助

摘　　要：在大鼠小脑脑片上观察了ＮＡ和５－ＨＴ对浦肯野细胞（ＰＣ）的自发放电活动及由白质刺激所引起的诱发放电活动的影响。结果表明：（１）ＮＡ使ＰＣ产生抑制、兴奋和双相反应，以抑制反应为主（７９．８％）；５－ＨＴ引起ＰＣ兴奋和抑制反应，以兴奋反应略多（５７．８％）。（２）先后灌流ＮＡ和５－ＨＴ对同一个ＰＣ自发放电的影响主要为抑制（ＮＡ）－兴奋（５－ＨＴ）（５３．８％）。（３）ＮＡ对ＰＣ的诱发复杂锋电位（ＣＳ）和简单锋电位（ＳＳ）反应，主要产生增强效应（５７．１％和６２．８％）；５－ＨＴ对ＰＣ诱发ＣＳ和ＳＳ反应则主要产生压抑作用（６０．０％和６８．２％）。（４）先后灌流ＮＡ和５－ＨＴ对同一个ＰＣ的诱发ＣＳ和ＳＳ反应，主要表现为ＮＡ对这两种诱发反应的增强和５－ＨＴ的压抑效应（６０．０％和５２．９％）。这些结果提示，ＮＡ能和５－ＨＴ能传入纤维可以通过释放ＮＡ和５－ＨＴ调节ＰＣ的兴奋性水平并改变ＰＣ对爬行纤维和苦状纤维突触传入的反应敏感性，影响小脑皮层神经元网络的感觉运动整合过程。Effects of NA and 5-HT perfusion on the spontaneous activity and evoked responses of the Purkinje cells (PCs) to stimulation of white matter were studied in the rat cerebellar slices. The results were as follows: (1) Perfusion with NA could elicit inhibitory, excitatory or biphasic responses of the spontaneously discharging PCs, with inhibiterry response in dominance (79. 8 % ). 5-HT perfusion could also exert either excitatoryor inhibitory effect on PC spontaneous activity. (2) When NA and 5-HT were perfused successively, NA tended to inhibit while 5-HT tended to excite in 53. 8% of thetested cells. (3) Most of the complex spike (CS) and simple spike (SS) responses evoked by white matter stimulation could be potentiated by NA (68. 2%, 57. 1 % ) ordepressed by 5-HT (60. 0 %, 68. 2 % ). (4 ) On the same cell under successively perfusion with NA and 5-HT, the evoked CS and SS responses of most cells (60. 0%,52. 9 % ) showed a tendency to be facilitated by NA and suppressed by 5-HT. These resuits suggest that aminergic afferent fibers, by releasing NA and 5-HT, may affectPC′s excitability and responsiveness to synaptic inputs from mossy and climbing fibers,thus playing an important role in the cerebellar function.

关 键 词：小脑 浦肯野细胞 去甲肾上腺素 血清素 电活动 

分 类 号：Q424[生物学—神经生物学] R338.23[生物学—生理学]