胰岛素样生长因子Ⅱ对大鼠离体培养黄体细胞孕酮生成的影响  

EFFECT OF IGF-II ON PROGESTERONE PRODUCTION BY CULTURED RAT LUTEAL CELLS IN VITRO

作　　者：倪江[1] 朱辉[1] 张玮[1] 王京花[1] 马青年[1] 

机构地区：[1]哈尔滨医科大学生理教研室

出　　处：《生理学报》1996年第5期507-511,共5页Acta Physiologica Sinica

摘　　要：本实验观察了胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）对大鼠离体培养黄体细胞孕酮生成的影响，并对其作用机制进行了探讨。结果显示，ＩＧＦ－Ⅱ能显著地促进大鼠离体培养黄体细胞孕酮生成并呈剂量－效应关系，同时还能促进３Ｈ－亮氨酸（３Ｈ－ｌｅｎ）掺入黄体细胞蛋白质的合成，促进３Ｈ－胸腺嘧啶（３Ｈ－ＴｄＲ）掺入ＤＮＡ的合成，而上述效应分别被放线菌酮（ＣＹＸ）和放线菌素Ｄ（ＡｃｔＤ）所抑制。此外，ＩＧＦ－Ⅱ对大鼠高体黄体细胞内源性ｃＡＭＰ和ｈＣＧ诱导的ｃＡＭＰ含量均无影响，这提示ＩＧＦ－Ⅱ对大鼠黄体细胞孕酮生成的促进作用与其增强黄体细胞蛋白质与ＤＮＡ的合成有关，而其跨膜信息传递似不通过ｃＡＭＰ系统。It has been reported recently that IGF- Ⅱplays an important role in the regulationof ovarian function. The aim of this study is to investigate whether IGF-Ⅱ can affectprogesterone production by cultured rat luteal cells. The results showed that IGF-Ⅱcould significantly stimulate basal progesterone production in a dose-dependant mannerwithout altering the cAMP content. IGF-Ⅱ could enhance 3H-len incorporation into protein and 3H-TdR incorporation into DNA of the luteal cells, but these effects could beinhibited by CYX and Act D. The above results suggest that stimulating effect of IGF Ⅱ on progesterone production by rat luteal cells may be related to enhanced protein andDNA synthesis.

关 键 词：胰岛素 生长因子Ⅱ 孕酮 黄体细胞 

分 类 号：R339.22[医药卫生—人体生理学] Q578[医药卫生—基础医学]