HPLC法测定野生与栽培藏药麻花艽中4种环烯醚萜苷类成分的含量  

HPLC simultaneous determination of four iridoid glycosides in wild and cultivated Gentiana straminea Maxim.

作　　者：孙菁[1,2] 陈桂琛[1] 李玉林[1,2] 索有瑞[1] 马玉花[3] 

机构地区：[1]中国科学院西北高原生物研究所 [2]中国科学院研究生院,北京100049 [3]中国科学院研究生院

出　　处：《药物分析杂志》2006年第10期1373-1376,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家中西部专项(2001BA901A47);中国科学院知识创新工程领域前沿项目(CXLY-2002-08)共同资助

摘　　要：目的:对野生和栽培藏药麻花艽中龙胆苦苷、落干酸、獐牙菜苦苷和獐牙菜苷4种苦苷类成分进行高效液相色谱的含量测定,并比较分析它们之间的差异。方法:采用 Eclipse XDB-C_8色谱柱(4.6mm×150mm,5μm),流动相 A 为95%乙腈水溶液,B 为5%乙腈(含10mmol·L^(-1)的甲酸)水溶液,A 在0～20min 内比例由0→100%进行线性洗脱,流速1.0mL·min^(-1),检测波长240nm,柱温30℃。结果:4种成分均达到基线分离,龙胆苦苷、落干酸、獐牙菜苦苷、獐牙菜苷的线性范围分别为0.60～19.20μg(r=0.9999),0.24～7.68μg(r=0.9999),0.38～12.02μg(r=0.9999),0.05～1.66μg(r=0.9999);回收率分别为99.73%,98.13%,98.45%,96.22%。结论:栽培藏药麻花艽中苦苷类成分的含量已经接近或超过野生种的水平,可初步代替野生药材入药。Objective：To determinate the contents of four iridoids glycosides, gentiopicroside, loganic acid, swertiamarin and sweroside in wild and cultivated Gentiana straminea Maxim. by HPLC. Method：The samples were separated on an Eclipse XDB - Ca column（4.6 mm × 150 mm,5 μm） with 95% acetonitrile （A） and 5% acetonitrile （10 mmol · L^-1 formic acid） （B） gradient elution with flow rate at 1.0 mL · min^-1. Mobile phase（A） increased from 0 to 100% in 20 min with UV detection at 240 nm and column temperature at 30 ℃. Results： Four compounds were isolated on base line. The linear ranges of iridoids, gentiopicroside, loganic acid, swertiamarin and sweroside were 0.60 - 19.20 μg（r =0. 9999） ,0. 24 -7.68 μg（r =0. 9999） ,0. 38 - 12.02 μg（r =0. 9999） ,0.05 - 1.66 μg（ r = 0. 9999 ） , respectively. The average recoveries were 99.73 % , 98.13% , 98.45 % and 96. 22% , respectively. Conclusions： The contents of four iridoid glycosides in cultivated Gentiana straminea have reached or exceeded those in the wild one. Therefore,it is preliminarily feasible for the cultivated G. straminea used as a herbal medicine to replace the wild one.

关 键 词：高效液相色谱 麻花艽 龙胆苦苷 落干酸 獐牙菜苦苷 獐牙菜苷 野生 栽培 

分 类 号：R917[医药卫生—药物分析学]