CO_2超临界流体萃取八角果壳和种子挥发油化学成分的GC-MS分析  

GC-MS analysis of essential oils from pericarp and seeds of Illicium verum Hook.f.extracted by supercritical CO_2 fluid

作　　者：袁经权[1] 冯洁[2] 杨峻山[3] 缪剑华[1] 

机构地区：[1]广西药用植物研究所,南宁530023 [2]北京大学药学院天然药物与仿生药物国家重点实验室,北京100083 [3]中国医学科学院-中国协和医科大学药用植物研究所,北京100094

出　　处：《药物分析杂志》2006年第10期1380-1384,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技攻关计划"西部开发"重大项目(2005BA901A09)

摘　　要：目的:分别研究八角果壳和种子挥发油化学成分,为综合利用八角提供科学依据。方法:采用 CO_2超临界流体分别萃取八角果壳和种子挥发油,GC-MS 法鉴定化学成分,归一化法测定各成分相对含量。萃取条件:压力20MPa,温度40℃;GC-MS 条件:DB-5石英毛细管色谱柱(0.25mm×30m,0.25μm);载气为高纯氦气(流速1.0mL·min^(-1))。质谱分析条件:EI电离源70eV;离子源温度220℃。结果:从果壳和种子挥发油中共鉴定出71个化合物,其中从果壳挥发油中鉴定出61个化合物,占果壳挥发油85.33%,主要成分为茴香脑(45.29%)、对丙烯苯基异戊烯醚(9.23%)和右旋柠烯(6.25%);从种子挥发油中鉴定出22个化合物,占种子挥发油84.92%,主要成分为茴香脑(58.04%)、顺式-2-葵烯醛(5.02%)和顺式-2-庚烯醛(4.73%)。果壳、种子特有化合物分别为49个和10个,果壳与种子共有化合物为12个;在鉴定出的71个化合物中,有26个化合物为从八角中首次发现。结论:八角果壳和种子挥发油的主要成分均为茴香脑,种子含量甚至比果壳高,需加大对八角种子的开发力度,并且它们次要成分不同,也应加以区别利用和有针对性地开发。Objective：To analyze the constituents of essential oil extracted from the pericarp and seeds of Illicium velum Hook. f. to establish foundation for reasonable utilization of them. Methods： The essential oils were extracted by CO2 supercritical fluid extraction （SFE- CO2 ） method, then separated and identified by GC -MS. Relative content of each component was determined by area normalization. Results ：61 components were identified from the pericarp, the identified compounds account for 85.33% of total oil in the pericarp;The main constituents in essential oil of the pericarp were transanethole （45.29%）, fenieuline （9. 23 % ）, D -limonene （6.25 % ）. 22 components were identified from the seeds, the identified compounds account for 84. 92% of total oil in the seeds;The main constituents in essential oil of the seeds were transanethole （58.04%）, （Z） -2 - decenal （5.02%）, （Z） -2 -heptenal （4. 73% ）. 49 compounds are only existed in the pericarp, 10 compounds are only existed in the seeds, 12 compounds are existed both in seeds and pericarp,26 compounds are identified from the plant for the first time. Conclusions：The pericarp and seeds of lllicium verum Hook. f. have almostly the same main compound, but their content and the secondary compounds are different, and should be treated differently.

关 键 词：八角 果壳 种子 CO2超临界流体萃取 挥发油 GC—MS 

分 类 号：R917[医药卫生—药物分析学]