6个微卫星座位与北京荷斯坦母牛乳成分性状关系的研究  

Relationships between Six Microsatellite Loci and Milk Composition Traits in Beijing Holstein Cows

作　　者：储明星[1] 王超[2] 李学伟[3] 叶素成[1] 方丽[1] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]聊城大学生命科学学院,聊城252059 [3]四川农业大学动物科技学院,雅安625014

出　　处：《农业生物技术学报》2006年第4期468-473,共6页Journal of Agricultural Biotechnology

基　　金：北京市自然科学基金资助项目(6002014和6022015)。

摘　　要：选择与奶牛乳成分性状紧密相关的6个微卫星座位BM711、BM143、BM1443、BM1905、BM415和BM4505,用非变性聚丙烯酰胺凝胶电泳分析其在200头北京荷斯坦母牛中的遗传变异。计算了其等位基因频率、杂合度、多态信息含量和有效等位基因数,并利用最小二乘法拟合线性模型初步探索了这6个微卫星座位与北京荷斯坦母牛乳成分性状的关系。结果表明:与乳脂率显著相关的最有利基因型在BM4505座位上为225bp/225bp,在BM415座位上为168bp/168bp,在BM711座位上为190bp/190bp;与蛋白率显著相关的最有利基因型在BM1905座位上为201bp/201bp,在BM1443座位上为163bp/163bp,在BM415座位上为168bp/168bp,在BM143座位上为110bp/110bp;与乳糖率显著相关的最有利基因型在BM1905座位上为201bp/201bp,在BM415座位上为168bp/168bp,在BM143座位上为108bp/108bp;与干物质率显著相关的最有利基因型在BM4505座位上为225bp/225bp,在BM415座位上为168bp/168bp。Genetic variation of six microsatellite loci BM4505, BM1905, BM1443, BM415, BMI43 and BM711 which were closely correlated with milk composition traits was analyzed in 200 Beijing Holstein cows by nondenaturing polyacrylamide gel electrophoresis. Allele frequency, heterozygosity, polymorphic information content, effective number of alleles of the six microsatellite loci were calculated. Relationships between six microsatellite loci and milk composition traits in Beijing Holstein cows were preliminarily analyzed by least squares linear model. The results indicated that microsatellite BM4505 significantly correlated with fat percentage and dry matter percentage; microsatellite BM1905 significantly correlated with protein percentage and lactose percentage; microsatellite BM1443 significantly correlated with protein percentage; microsatellite BM415 significantly correlated with fat percentage, protein percentage, lactose percentage and dry matter percentage; microsatellite BM143 significantly correlated with protein percentage and lactose percentage; microsatellite BM711 significantly correlated with fat percentage. 225 bp/225 bp at BM4505, 168 bp/168 bp at BM415 and 190 bp/190 bp at BM711 were the most favourable genotypes for fat percentage. 201 bp/201 bp at BM1905, 163 bp/163 bp at BM1443, 168 bp/168 bp at BM415 and 110 bp/110 bp at BMI43 were the most favourable genotypes for protein percentage. 201 bp/201 bp at BM1905, 168 bp/168 bp at BM415 and 108 bp/108 bp at BMI43 were the most favourable genotypes for lactose percentage. 225 bp/225 bp at BM4505 and 168 bp/168 bp at BM415 were the most favourable genotypes for dry matter percentage.

关 键 词：荷斯坦母牛 乳成分性状 微卫星 遗传多态性 

分 类 号：S823.91[农业科学—畜牧学]