山羊骨髓MSCs体外向成骨细胞诱导和分化  

The Osteoblast Differentiation In vitro of Goat Marrow Mesenchymal Stem Cells(MSCs)

作　　者：庞全海[1] 窦忠英 

机构地区：[1]国家干细胞工程技术研究中心陕西分中心

出　　处：《农业生物技术学报》2006年第5期683-687,I0001,共6页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究规划(973)项目(No.G1999054301-5);国家高技术研究与发展计划(863)项目(No.2002AA216161);国家自然科学基金项目(No.39970363);教育部重点专项(No.03160);山西省科技攻关项目(No.021044)资助

摘　　要：以关中奶山羊骨髓间充质干细胞(MSCs)为对象,研究了体外诱导山羊MSCs向成骨细胞的分化。结果表明,山羊MSCs在地塞米松、β-磷酸甘油和抗坏血酸的作用下,从诱导的第4￣5天开始,即可见有些MSCs体积逐渐增大,由梭形或多角形转变为立方形或卵圆形;随着诱导时间延长,立方形或卵圆形细胞不断增多,到第15天时,有较多的立方形细胞,且这种变化经历了从立方形向卵圆形转变的过程;第25天,可见卵圆形和方形细胞堆积,诱导分化率为90.2%±2.97%。说明其被诱导分化为成骨细胞表型,该类细胞呈茜素红染色和碱性磷酸酶(ALP)染色阳性。从而证明了山羊骨髓MSCs具有体外分化为成骨细胞的潜能,从而为利用该类细胞作为实验模型研究骨骼缺损修复等提供了科学依据。The mesenchymal stem ceils （MSCs） derived from bone marrow of the adult Guanzhong dairy goats were used to be induced.to differentiate into osteoblast phenotype in vitro. The results showed that, after induced for 4-5 d by dexamethasone, beta-glycerophosphate and ascorbate-2 phosphate, the volume of some cells of the goat MSCs gradually largened and changed their form from spindle/polygon to cuboid/ovoid; With the induce time continuing, the numbers of the cuboid/ovoid ceUs continuous increased; At 15th day, there were many cuboid cells, and the modality of the cells changed from cuboid to ovoid; At 25th day, the oval and cuboid cells accumulation were observed. And the osteoblasts differentiation rate of the cells was 90.2% ± 2.97%. These indicated that the goat MSCs were induced to differentiate into osteoblast phenotype and the new born cells demonstrated positive staining for alizarin red and alkaline phosphatase （ALP）. These results suggested that the MSCs were induced to differentiate into osteoblst phenotype in vitro. Accordingly, the data can provide a scientific foundation with cellular mode and further study for the bone defaults repairing.

关 键 词：山羊骨髓间充质干细胞(MSCs) 体外 诱导 成骨分化 

分 类 号：S184[农业科学—农业基础科学]