鼠疫耶尔森菌LcrV抗原B细胞表位的预测  

Prediction of B cell epitopes for LcrV from Yersinia pestis

作　　者：王文敬[1] 李明[1] 董文其[1] 

机构地区：[1]南方医科大学生物技术学院,广州510515

出　　处：《中国地方病学杂志》2006年第6期593-595,共3页Chinese Jouranl of Endemiology

基　　金：国家973项目基金资助(2002CB513206)

摘　　要：目的预测鼠疫耶尔森菌LcrV抗原的B细胞表位,为抗LcrV单克隆抗体的制备提供理论依据,探索适合我国鼠疫耶尔森菌蛋白B细胞表位组研究的可行方案。方法采用EMBOSS网络平台以及DNAStar Protean软件分析LcrV蛋白的二级结构、可塑性、亲水性和抗原性指数等参数,进行B细胞表位的预测和分析。结果LcrV蛋白的B细胞表位可能位于第16～24、26～31、45～50、175～181、208～213和280～289位等氨基酸区域内或附近,其中16～24、26～31、175～181、208～213等4个短肽都与文献报道相吻合。结论预测得到的表位为后续抗LcrV抗原单克隆抗体的制备奠定了理论基础,为进一步表位分析和鉴定提供了参考依据;预测结果的准确性高,有助于利用表位预测法建立鼠疫菌蛋白B细胞表位研究的平台。Objective To predict the B cell epitopes for LcrV antigen and provide theoretical references for preparing monoclonal antibodies against LcrV, and to exploring a feasible method or protocols for studying B cell epitomics of Yersinia pestis in China. Methods Amino acid sequence of LcrV on secondary structure, LcrV flexibility and hydrophilicity and antigenic index were analyzed using DNAStar Protean softeware and EMBOSS net platform to predict the B cell epitopes. Results B cell epitopes of LcrV located in or adjacent to amino acids 16 - 24,26 31,45 - 50,175 - 181,208 - 213 and 280 - 289, among which 4 short peptides of 16 - 24,26 - 31,175 - 181 and 208 - 213 were consistent with literature reports. Conclusions The prediction of B cell epitopes provides theoretical basis for producting monoclonal antibodies against LcrV and provide references for analysis and identification of B cell epitopes. Precise prediction helps for establishing research platform of epitomics on Yersinia pestis.

关 键 词：LcrV抗原 耶尔森菌 鼠疫 表位 B淋巴细胞 预测 

分 类 号：R392[医药卫生—免疫学]