辣根过氧化物酶标记不同表型鼠疫F1单克隆抗体的效果观察  

Observation on effect of four plague F1 antibodies labeled with horseradish peroxidase using improved method of NaIO_4

作　　者：尹家祥[1] 唐雪[1] 陈平[1] 杜春红[1] 董兴齐[1] 于国林[1] 

机构地区：[1]云南省地方病防治所,大理671000

出　　处：《中国地方病学杂志》2006年第6期596-597,共2页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(30160078);云南省科技攻关计划项目(2002NG17)

摘　　要：目的观察用辣根过氧化物酶(HRP)标记4种鼠疫F1抗体的效果。方法使用过碘酸钠改良法制备酶结合物,酶标抗体的效价和最适工作浓度用双抗体夹心法测定。结果3株鼠疫F1单克隆抗体中B12标记成功;鼠疫γ球蛋白标记效果较鼠疫F1单克隆抗体B12差。结论过碘酸钠改良法制备辣根过氧化物酶标记鼠疫F1单克隆抗体B12效果优于另外3种鼠疫F1抗体。Objective To observe the effect of four plague F1 antibodies against F1 antigen labeled with horseradish peroxidase （HRP）. Methods HRP-labeled anti-F1 antibody was prepared using the improved method of NalO4. The titre and the optimal working dilution of labeled-F1 antibody were determined by double-antibody sandwich ELISA method. Results Among three HRP-labeled plague F1 monoclonal antibodies （McAb）, B12 F1 McAb was successfully labeled with HRP. The effect of HRP-labeled plague B12 F1 McAb was better than that of HRP-labeled plague γ IgG. Conclusions The effect of HRP-labeled plague B12 F1 McAb using the improved method of NalO4 is better than another 3 HRP-labeled plague F1 antibodies.

关 键 词：鼠疫 F1抗原 单克隆抗体 过碘酸钠改良法 

分 类 号：R392[医药卫生—免疫学]