西藏大骨节病患者血清透明质酸、一氧化氮和硒水平检测分析  

The changes of hyalurona, nitric oxide and selenium contents in serum for patients with Kashin-Beck disease in Tibet

作　　者：康龙丽[1] 许鹏[2] 扎西桑珠 巴桑卓玛 李小迎[2] 郭雄[2] 

机构地区：[1]西藏民族学院生命科学基础实验室,西藏高原环境与疾病相关实验室,咸阳712082 [2]西安交通大学教育部环境与疾病相关基因实验室,环境与地方病研究室 [3]西藏地方病研究所

出　　处：《中国地方病学杂志》2006年第6期694-696,共3页Chinese Jouranl of Endemiology

基　　金：西藏自治区科技厅重点课题资助项目(2005-56);西藏民族学院基金资助项目(05MY11)

摘　　要：目的测定西藏成人大骨节病患者血清透明质酸(HA)、一氧化氮(NO)和硒(Se)水平的变化,探讨HA、NO和Se在西藏大骨节病发生发展的机理。方法在西藏拉萨大骨节病病区随机选取大骨节病患者30例(大骨节病组)、病区内健康者30例(病区内对照组)、非大骨节病病区健康者30例(病区外对照组),3组人群年龄、性别无显著差异。采取静脉血离心制备血清,用酶联免疫吸附试验(ELISA)检测HA、硝酸还原酶法测定NO、荧光法测定血清Se水平。结果①大骨节病组、病区内、外对照组血清HA水平分别为(68．56±8．34)、(90．55±18．25)、(92．18±14．61)μg/L,大骨节病组血清HA水平显著低于病区内、外健康对照组(P<0．05);②3组血清NO水平分别为(102．98±26．15)、(35．73±13．32)、(34．65±12．21)μmol／L,大骨节病组血清NO水平显著高于病区内、外健康对照组(P<0．05);③3组血清Se水平分别为(73．34±22．72)、(68．76±20．56)、(98．73±37．20)μg／L,病区人群血清Se水平显著高于非病区人群(P<0．05);④血清HA水平与血清Se水平呈显著正相关(r=0．196,P<0．05),血清Se水平与NO水平有负相关趋势,但差异无统计学意义(r=～0．085,P>0．05)。结论西藏成人大骨节病的发生发展与血清HA水平降低和NO水平升高有关。Objective To investigate the relation between contents of serum hyaluronate acid （HA）, nitric oxide（NO）, and selenium（Se） of patients with Kashin-Beck disease（KBD） and the disease activity in Tibet Lhasa. Methods 90 subjects, from KBD areas and non-KBD areas of Lhasa city. Tibet Province, were divided into 3 groups： the group with KBD（30）, the control in KBD areas（30）, the control in non-KBD areas（30）. The sex and the average age in the three groups had no difference. The contents of HA, NO and Se were detected by ELISA, nitrifying ferment, and method of atomic fluorescent spectrometer, respectively. Results ①The HA contents of serum in three groups were（68.56 ± 8.34）, （90.55 ± 18.25） and （92.18 ± 14.61 ）μg/L. The contents of HA in serum of the groups with KBD were significantly lower than that the control in KBD areas and non-KBD areas （P 〈 0.05）. ②The NO contents of serum in three groups were（ 102.98 ± 26.15）, （35.73 ± 13.32） and（34.65 ± 12.21 ）μmoL/L, respectively. The contents of NO in serum of the group with KBD were significantly higher than those of the controls in KBD areas and non-KBD areas （P 〈 0.05）. ③The Se contents of serum in three groups were （73.34± 22.72）, （68.76 ± 20.56） and （98.73 ± 37.20）μg/L, respectively. The contents of Se in serum of the groups living in KBD areas were significantly lower than that of the control in non-KBD areas （P 〈 0.05）. ④There was a positive relation between Se and HA contents in serum （r = 0.196,P 〈 0.05）, and a negative relation trend between Se and NO contents in serum （r = - 0.085 ,P 〉 0.05 ）. Conclusions The decrease of HA and Se contents ,the increase of NO content in serum may relate to the pathogenesis of KBD.

关 键 词：大骨节病 透明质酸 一氧化氮 硒 

分 类 号：R684.1[医药卫生—骨科学]