正常成年大鼠神经元前体细胞的迁移  

MIGRATION OF NEURONAL PRECURSORS IN NORMAL ADULT RAT

作　　者：谭新杰[1] 胡长林[2] 蔡文琴[3] 

机构地区：[1]贵州省人民医院干部医疗科,贵阳550002 [2]重庆医科大学附属第二医院神经内科,重庆400010 [3]第三军医大学基础部神经生物学教研室,重庆400038

出　　处：《解剖学报》2006年第5期495-497,共3页Acta Anatomica Sinica

摘　　要：目的探讨正常成年大鼠脑中神经元前体细胞的迁移路径和迁移特征,为病理状况下的神经元迁移研究提供理论和实验基础。方法将成年大鼠脑组织按冠状位和矢状位行冰冻切片(片厚20μm),采用免疫组织化学染色技术观察Doublecortin(DCX)免疫阳性细胞的迁移路径以及DCX阳性细胞的迁移特征。结果正常成年大鼠神经元前体细胞的迁移主要有两条路径:一条是从室下区到嗅球的吻侧迁移流,另一条是位于皮层和胼胝体之间的胼胝体周围迁移流。吻侧迁移流中的DCX免疫阳性细胞较规则的有序排列,形态上呈梭形的胞体和单个较长的前导突起,前导突起基本朝向嗅球,细胞大小基本一致:而胼胝体周围迁移流中的DCX免疫阳性细胞胞体呈类圆形,突起的大小、数目和方向多样。结论研究神经元前体细胞的迁移不仅有助于明确其迁移的具体机制,而且有助于控制神经元的迁移和设计有效的治疗策略。Objective To investigate the migration pathway and feature of neuronal precursors in normal adult rats and provide theory and experiment basis for the neuronal migration under pathological condition. Methods The adult rat brains were cut into 20 μm coronal and sagittal sections on a freezing microtome. Immunohistochemistry was applied to observe the migration pathway and feature of DCX-expressing cells. Results There were two migration pathways with the neuronal precursors in normal adult rats. One was the rostral migratory stream （RMS） from the subventricular zone to the olfactory bulb, another appeared to travel in a chain along the interface between cortex and corpus callosum. The DCX-positive cells in the RMS had the fusiform somata with a single leading process and the process orientated to the olfactory bulb, while the DCX-positive cells around the corpus callosum had similarly round somata and the size, number and orientation of process were of diversity. Conclusion The study of neuronal precursors migrating not only contributes to identify the migration mechanisms, hut also contributes to the control of neuronal migration and designs some effective therapy strategies.

关 键 词：神经元前体细胞 迁移 免疫组织化学 大鼠 

分 类 号：R338.1[医药卫生—人体生理学]