外源性已烯雌酚对新生雌性BALB/c小鼠脾内细胞雌激素受体表达的影响  

DYNAMIC EFFECT OF ADMINISTERING DIETHYLSTILBESTROL EXTERNALLY TO NEONATAL FEMALE BALB/c MICE ON THE EXPRESSION OF ESTROGEN RECEPTOR IN MICE SPLEENS

作　　者：徐彩红[1] 张京[1] 卫兰[1] 马强 

机构地区：[1]北京大学基础医学院组织学与胚胎学教研室,北京100083 [2]北京天坛生物制品股份有限公司,北京100024

出　　处：《解剖学报》2006年第5期573-577,共5页Acta Anatomica Sinica

摘　　要：目的了解新生期注射己烯雌酚(DES)后雌性BALB/c小鼠脾内细胞雌激素受体表达的动态变化。方法雌性BALB/c小鼠在生后24h内,颈背部皮下注射DES,每隔24h注射1次,共连续注射5次。对照组平行注射等量无菌豆油。分别在生后7、14、21、35和49d将小鼠处死,取其脾组织进行常规石蜡包埋、切片及雌激素受体(ER)的免疫组织化学检测。结果对照组与DES组雌性小鼠脾内均可见ER阳性细胞,ER阳性细胞主要是淋巴细胞,其阳性强度在两组均呈增龄性变化;DES组小鼠脾内ER阳性细胞强度高于对照组,两组之间存在显著性差异。结论雌性BALB/c小鼠脾内存在表达ER的细胞,其表达水平随着年龄的增加而有所增加;新生期接触DES可以导致雌性BALB/c小鼠脾ER阳性细胞的增加,并可至少持续到成年期。Objective To investigate the dynamic changes of estrogen receptors （ER） in spleen of female BALB/c mice after injected with diethylstilbestrol （DES） beginning in neonatal period. Methods Neonatal female BALB/c mice were injected subcutaneously on cervix - backside with DES within 24 hours after birth at intervals of 24 hours for 5 times. Control groups were injected with olive oil with the same method. Mice were killed on 7 days, 14 days, 21 days, 35 days and 49 days after birth separately, the spleen was taken out, imbedded with paraffin and sectioned serially in 5μm. The expression of ER in slice of spleen was detected by immunohistochemistry technique. Results ER positive cells were present in both DES-injected groups and control groups. ER positive cells were mostly lymphocytes. A correlation between age and the intensity of positive reactions was showed in both groups. The intensity of ER immunohistochemistry positive reaction in DES group was much stronger than that in control group, and there was a significant statistic difference between the two groups. Conclusion The ER expression exists in spleen of female BALB/c mice and increases with the age of mice. The contact of DES in neonatal female BALB/c mice will lead to an increased ER expression in their spleens and this effect can continue to their manhood at least.

关 键 词：己烯雌酚 脾 雌激素受体 免疫组织化学 新生BALB/c小鼠 

分 类 号：R392[医药卫生—免疫学]