时间分辨荧光免疫分析技术在检测乙肝标志物中的应用评价  

Estimation of Time-Resolved Fluorescence Immunoassay on Detection of Serum Markers of Hepatitis B Virus

作　　者：李瑞炎[1] 熊金虎[1] 余晓萍[1] 

机构地区：[1]武汉大学中南医院,武汉430071

出　　处：《武汉大学学报（医学版）》2006年第6期797-799,802,共4页Medical Journal of Wuhan University

摘　　要：目的:评价时间分辨荧光免疫分析(TRFIA)技术在检测乙肝标志物中的应用价值。方法:采用TRF法对5项乙肝标志物检测,并同时进行微粒子酶免技术(MEIA)及ELISA法检测并统计分析。结果:乙肝标志物5项指标的日间变异系数均在5%以下,批内精密度的变异系数<5%,线性测定相关系数为1,各组理论值与实测值之间无差异。靶值与实测值有统计学意义(P<0.01)。乙肝标志物5项指标的携带互污染率小于1.5%,3种方法检测结果临床符合性一致。结论:TRF分析仪采用TRF法对乙肝标志物5项的检测中,TRF法灵敏度高,是一种比较理想的定量检测方法。Objective： To analyze the value of the time resolved fluorescence immunoassay （TRFIA） on detecting the immune markers of hepatitis B virus. Methods： By TRF, the study detected five immune markers of hepatitis B virus comparing with those detected by the methods of micro particle enzyme immunoassay （MEIA） and ELISA. Results： The variance ratios of immune markers of hepatitis B virus were below 5 %, the variance ratios of precision were below 5%, and all linear correlative coefficients between theoretical and practical values were 1 in all the five serum HBV markers. The mutual pollution rates of immune markers of viral B hepatitis were below 1.5 %. All the results by the three methods accorded with the clinic diagnosis. Conclusion： TRF is a perfect quantitative method in detecting hepatitis B virus.

关 键 词：时间分辨荧光免疫分析 乙肝免疫标志物 乙肝定量 

分 类 号：R446.61[医药卫生—诊断学]