用于鸡生长和肉质性状定位资源群体的构建  

Construction of Resource Populations for Mapping Chicken Growth and Meat Quality Traits

作　　者：罗成龙[1] 张德祥[1] 徐海平[1] 彭志军 何丹林[1] 曾华[1] 聂庆华[1] 梁少东[1] 杨关福[1] 张细权[1] 

机构地区：[1]华南农业大学动物科学学院,广州510640 [2]广东温氏食品集团有限公司,新兴527400

出　　处：《畜牧兽医学报》2006年第11期1099-1106,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展规划项目(G2000016102);广东省农业攻关项目(C20704)

摘　　要：采用远交F-2设计,以杏花鸡(A系)为亲本,分别与隐性白洛克鸡(B系)、泰和丝羽乌骨鸡(C系)进行全同胞和半同胞的正反交,产生了包含A♂×B♀、B♂×A♀、A♂×C♀、C♂×A♀4个杂交组合的各两组F2群体,并进行详细的性状表型记录,建立了可用于定位生长和肉质的数量性状座位(quantitative trait loci,QTL)的4个资源群体。对F2群体的各种性状表型值进行分析,结果显示,F2群体各生长性状和肉质性状都分离得很好。资源群体有足够的群体规模,并有利于校正母体效应和环境影响,保证有效单核苷酸多态性(single nucleotide poly-morphism,SNP)检测数量和QTL定位的准确性,使资源群体的构建达到了预期的效果。F2 resource populations, consisting of 4 branch families, were constructed from reciprocal out-crossings of Xinghua chickens （Line A）, White Recessive Rocks （Line B） and Taihe Silkies （Line C）, in which the mating of male line A and female line B, male line B and female line A, male line A and female line C, male line C and female line A produced full-sib and half-sib individuals, with outbred F-2 design. From then growth performance and meat quality traits in F2 individuals were measured and depicted by stages. The analysis of phenotypic values indicated that the measured traits on growth performance and meat quality were segregating well in F2 individuals. The population size is large enough, and the resource population can correct maternal and environmental effects. It can promise the scale and density of single nucleotide polymorphisms and the accuracy of QTL mapping. Therefore, the construction of resource populations achieved the expected aim.

关 键 词：鸡 生长性状 肉质性状 资源群体 QTL 

分 类 号：S831.2[农业科学—畜牧学]