民猪、长白猪及其杂种母猪催乳素受体(PRLR)基因的Nae I多态性与繁殖性状的相关分析  

Nae I Polymorphism of PRLR Gene and Its Effect on Reproductive Traits in Minpig,Landrace and Their F_1 Crossbreed Sows

作　　者：胡雪松[1] 王希彪[1] 

机构地区：[1]东北农业大学动物科技学院

出　　处：《畜牧兽医学报》2006年第11期1135-1140,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省科技攻关计划项目资助课题(GC03B509)

摘　　要：采用测序,结合PCR-RFLPs的方法在民猪、长白猪及其杂种母猪的催乳素受体(Prolactin Receptor,PRLR)cDNA序列(+1 628^+1 878)中首次发现一处NaeI多态位点。该位点的多态性是由于第1 789位点的碱基发生了A→G的突变,并导致氨基酸的变化(S→G),即由丝氨酸→甘氨酸的变化。分析结果表明,NaeI酶切位点的基因型频率和等位基因频率在3个母猪群体间有显著差异(P<0.01)。最小二乘分析结果显示,在民猪群体中,只存在AA和AB两种基因型,AB型母猪的初生窝重和21日龄仔猪均匀度显著高于AA型(P<0.05);在长白猪群体中,AA型母猪的21日龄窝重显著高于BB型(P<0.05);在F1代长民杂种猪群体中,BB基因型母猪的21日龄仔猪均匀度显著高于AB和AA型(P<0.05)。The prolactin receptor （PRLR） plays a key role in many reproductive pathways of animals. It is probable that the change of the structure of PRLR gene will lead to variation of PRLR function, and hence affect reproductive phenotype. In this experiment, the Nae Ⅰ polymorphism of PRLR gene and its effect on reproductive traits in Minpig, Landrace and their F1 crossbreed sows was studied by sequencing and PCR-RFLPs. A mutation of A to G （1 789） that resulted in a change of amino acid （S→G）, i.e. Serine→ Glycine was detected in cDNA sequence （＋1 628- ＋1 878） of PRLR gene. The frequencies of genotypes and alleles for Nae I polymorphism site were significantly different in three populations （P〈0.01）. The results of correlation analysis indicated that PRLR gene was closely associated with several reproductive traits examined. As regards Minpig, only two genotypes were detected, sows with AB genotype had significantly heavier litter weight at birth and higher piglets uniformity on day 21 than sows with AA genotype （P 〈0. 05）. For Landrace, sows with AA genotype have a greater litter weight on day 21 than sows with BB genotype （P〈0.05）. In the F1 （L× M）crossbreed population, piglets uniformity on day 21 was significantly higher for sows with BB genotype than sows with AB and AA genotypes （P 〈0. 05）.

关 键 词：民猪 PRLR基因 多态性 繁殖性能 长白猪 

分 类 号：S828.2[农业科学—畜牧学]