毒害艾美耳球虫初次感染雏鸡免疫器官IL-2和淋巴细胞增殖功能变化  

Changes of Proliferative Responses and Interleukin-2 Activity of Lymphocytes in Immune Organs of Chickens Primary Infected with E.Necatrix

作　　者：郑世民[1] 胡京友[1] 刘晶[1] 高雪丽[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《畜牧兽医学报》2006年第11期1222-1225,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省自然科学基金(C0206)

摘　　要：应用组织匀浆涂片和酸性α-醋酸萘酯酶（ANAE）染色及细胞培养技术和四甲基偶氮唑盐（MTT）测定法对毒害艾美耳球虫（eimeria necatrix，E．necatrix）初次感染雏鸡免疫器官的T细胞比例、白细胞介素-2（interleukin-2，IL-2）诱生活性、T细胞和B细胞对ConA或PMA的增殖功能的动态变化进行了较全面系统的研究。结果发现，E．necatrix初次感染雏鸡，其胸腺和脾脏T细胞比例分别于感染后7～21d和7～24d明显高于对照雏鸡；IL-2诱生活性分别于感染后16～18d和18～21d较对照雏鸡显著升高；T细胞对ConA的增殖反应分别在感染后14～16d和10～18d明显增加。法氏囊和脾脏B细胞对PMA的增殖反应分别于感染后14～24d和14～21d显著高于对照雏鸡。表明E．necatrix初次感染雏鸡免疫器官的IL-2调节及细胞免疫和体液免疫功能均明显提高。Dynamic changes of percentage of T cell,interleukin-2（IL-2） activity and proliferative response of T and B lymphocytes to concanavalin A（ConA） or phorble myristate acetate（PMA） in immune organs of 14-day-old chickens primary infected with E. necatrix were studied by using acid nonspecific α- naphthyl acetate esterase（ANAE）, cellular culture technique and [-3-（4,5-dimethylthiazol-2-yl）]-2,5-diphenyltetrazolium bromide （MTT） method. The results showed that the IL-2 activity in thymus and spleen of chickens primary infected with E. necatrix was significantly increased compared with that of control, respectively, at day 16-18 and 18-21 post primary infection （PPI） ; The proliferative responses of T lymphocytes to ConA in thymus and spleen of chickens infected with E. necatrix were significantly elevated compared with that of control chickens at day 14-16 and 10-18 PPI, respectively. The proliferative responses of B lymphocytes to PMA in spleen and bursa of Fabricius of chickens infected with E. necatrix were significantly higher than that of control at 14-21 and 14-24 PPI, respectively. These results showed that IL-2 immune regulation and function of cellular immune and fluid immune in immune organs of chickens primary infected with E. necatrix had significantly increased.

关 键 词：毒害艾美耳球虫 雏鸡 免疫器官 白细胞介素-2 T细胞 B细胞 

分 类 号：S855.9[农业科学—临床兽医学]