生物素对肉仔鸡脾脏细胞PCNA基因的mRNA表达的影响  

Effect of Biotin on mRNA Express of PCNA Gene in Speen Cell for Broilers

作　　者：于会民[1] 蔡辉益[1] 马书宇[1] 陈宝江[2] 

机构地区：[1]中国农业科学院饲料研究所,北京100081 [2]河北农业大学动物科技学院,保定071001

出　　处：《畜牧兽医学报》2006年第11期1232-1235,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：采用分子生物学方法研究了生物素对脾脏细胞增殖细胞核抗原(PCNA)基因的mRNA表达的影响。将60只1日龄AA雄性肉仔鸡随机分成3个处理,每个处理20只鸡,基础日粮为小麦-酪蛋白-鱼粉型日粮,在基础日粮中分别添加0.30 mg/kg和2.7 mg/kg 2个水平的生物素,包括对照组共计3种日粮处理,为了刺激PCNA基因表达产物mRNA的表达,在48日龄采用皮下注射方式定量接种禽流感疫苗。结果表明:添加生物素显著提高了脾脏细胞PCNA基因表达产物mRNA的表达量(P<0.01),其中2.7 mg/kg处理组极显著高于对照组和0.3mg/kg处理组(P<0.01),0.3 mg/kg处理组和对照组之间无显著性差异(P>0.05),由此推断,生物素通过促进脾脏细胞PCNA基因的mRNA表达,进而促进脾脏细胞的增殖。Molecular biological method was applied to explore the effect of biotin on rnRNA expression of PCNA gene in spleen, aimed to probe the mechanism of biotin enhancing spleen cell propagation. 60 AA male broilers of 1 day were allocated into 3 treatments, 20 broilers per treatment. The basal diet was wheat-casein-fish meal diet. Biotin was added in the basal diet at 0. 3 mg/kg and 2. 7 mg/kg. There were 3 diets including the control of basal diet. In order to stimulate rnRNA expression of PCNA gene, The avian flu vaccine was injected quantificationally and hypodermically into broilers on day 48. The results showed that addition of biotin elevated significantly rnRNA expression level of PCNA gene in spleen （P d0.01）, The rnRNA expression level of 2.7 mg/kg treatment was higher significantly than that of the control and 0. 3 mg/kg treatment （P〈0. 01）, and no significant difference was founded between 0. 3 mg/kg treatments and the control group. （P〉0. 05）. It was concluded that biotin can elevate rnRNA expression level of PCNA gene, thus enhance the propagation of spleen cell.

关 键 词：肉仔鸡 生物素 PCNA MRNA表达 

分 类 号：S831.5[农业科学—畜牧学]