干扰素-γ对妊娠早期大鼠下丘脑中胰岛素样生长因子-Ⅰ表达及妊娠结局的影响  

Effects of IFN-γ on the expression of IGF-Ⅰ gene in hypothalamus of pregnant rats and on their pregnant results

作　　者：司丽芳[1] 赵瑾[1] 贾利云[1] 许勃[1] 曹力[1] 乔海莲[1] 张艳[1] 范光丽[1] 

机构地区：[1]西北农林科技大学动物科技学院

出　　处：《中国兽医科学》2006年第11期920-923,共4页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(39670550);西北农林科技大学研究生科技创新基金资助项目(05ych020)

摘　　要：将妊娠9 d的大鼠随机分为对照组、试验1组、试验2组,采用生殖道肌肉注射的方法分别注射生理盐水、2.5×104U干扰素-γ(IFN-γ)、7.5×104U IFN-γ,采用免疫组织化学SP法研究IFN-γ对妊娠早期大鼠下丘脑中胰岛素样生长因子-Ⅰ的表达和妊娠结局的影响。结果显示,IGF-Ⅰ在下丘脑视前室周核、视上核、视前大细胞核、视前内侧核、视前外侧核均具有较多数量的阳性神经元表达;阳性细胞呈圆形、卵圆形、三角形,阳性物质大多位于细胞质和细胞核;生殖道肌肉注射IFN-γ后能下调IGF-Ⅰ在下丘脑的表达。同时,观察到试验2组妊娠大鼠子宫黏膜表面有大量紫褐色吸收斑。提示IFN-γ对妊娠早期大鼠IGF-Ⅰ在下丘脑的表达具有调节作用,且外源注射7.5×104U的IFN-γ后,会造成妊娠早期大鼠流产。In order to investigate effects of IFN-γ on the expression of IGF-Ⅰ gene in hypothalamus of pregnant rats and on their pregnant results, earlier pregnant rats were divided into the control group, experimental group 1 and experimental group 2. The control group was given with physiological brine and the two experimental groups were injected with 2.5×10^4 U of IFN-γ and 7.5× 10^4 U of IFN-γ by vaginal muscular, respectively. In results, IGF-Ⅰ were found in nucleus pre-opticus medianus, nucleus supraopticus, nucleus pre-opticus mango cellularis, nucleus pre-opticus medialis and nucleus pre-opticus lateralis of hypothalamus. Positive neurons were round, oval round and triangle, which were distributed mainly in plasma and nucleus after injection. The expression of IGF-Ⅰ gene in the hypothalamus was down-regulated, and a lot of purplish red dots were observed in the uterus membrane of the pregnant rats in the experimental group 2. The results suggested that the IFN-γ could regulate the expression of the IGF-Ⅰ gene in the hypothalamus, and 7.5 × 10^4 U of IFN-γ might cause abortion in earlier periods.

关 键 词：下丘脑 免疫组织化学 大鼠 干扰素-Γ 胰岛素样生长因子-Ⅰ 

分 类 号：S859.797[农业科学—临床兽医学] O786[农业科学—兽医学]