大豆雄性不育突变体NJ89-1核不育基因的SSR标记和定位  

SSR MARKER LOCATION OF MALE STERILE GENE OF NUCLEAR MALE STERILE MUTANT NJ89-1 IN SOYBEAN

作　　者：杨守萍[1] 曾维英[1] 段美萍[1] 喻德跃[1] 盖钧镒[1] 

机构地区：[1]南京农业大学国家大豆改良中心作物遗传与种质创新国家重点实验室,南京210095

出　　处：《大豆科学》2006年第4期344-348,共5页Soybean Science

基　　金：国家973项目(编号:2004CB7206);国家自然科学基金重大项目(编号:30490250);长江学者和创新团队发展计划(PC-SIRT);南京农业大学作物遗传与种质创新国家重点实验室开放基金课题(编号:ZW2004009)

摘　　要：组合NJ89-1不育株×南农73-935的F1、F1∶2、F2∶3的遗传数据表明在组合NJ89-1不育株×南农73-935中NJ89-1雄性不育性仍受一对隐性基因控制;随机选取组合NJ89-1不育株×南农73-935的一个F1∶2株行群体作为分子标记定位群体,对NJ89-1不育基因进行SSR标记定位,结果发现Sat-402与NJ89-1不育基因连锁,参照Song等(2004)整合的大豆遗传连锁图谱,初步将NJ89-1不育基因定位于大豆C2遗传连锁群上,NJ89-1不育基因与Sat-402的遗传距离为9.6cM。The analysis of genetic data of F1, F1：2 and F2：3 of cross NJ89- 1 sterile plant × Nannong73 （935 showed that the male sterility of NJ89-1 was still controlled by a single recessive nuclear gene. One population of a F2 line of cross NJ89-1 sterile plant X Nannong73（935 was selected randomly to map the male sterile gene of NJ89-1 through SSR markers. The results showed that Sat-402 was linked to the male sterile gene of NJ89-1. According to the integrated soybean genetic linkage groups of Song et al. （2004）, it was found that Sat-402 was on the linkage group C2. Thus the male sterile gene of NJ89- 1 was preliminarily located on the linkage group C2. The linkage distance between Sat-402 and the male sterile gene of NJ89-1 was 9.6cM.

关 键 词：大豆 雄性不育基因 SSR标记定位 

分 类 号：S565.1[农业科学—作物学]