海绵共附生放线菌Saccharopolyspora sp.nov中抗肿瘤活性成分的研究  

Antitumor active components of Saccharopolyspora sp.nov derived from Mycale plumose sponge

作　　者：刘睿[1] 方玉春[1] 段琳[1] 杜林[1] 朱天骄[1] 刘红兵[1] 顾谦群[1] 朱伟明[1] 

机构地区：[1]中国海洋大学海洋药物与食品研究所教育部海洋药物重点实验室,青岛266003

出　　处：《中国抗生素杂志》2006年第11期646-649,共4页Chinese Journal of Antibiotics

基　　金：国家863计划(2003A624020);国家自然科学基金(No.30472136&30470196)

摘　　要：羽毛山海绵(My ca le p lum ose)来源的糖多孢菌(S accharop oly sp ora sp.nov SP 2-10)具有诱导肿瘤细胞坏死的活性。本文对其发酵产物的活性部位乙酸乙酯层进行活性追踪分离,共得到7个化合物;利用理化性质和波谱学方法鉴定它们的结构分别为胆甾醇、邻苯二甲酸二异丁酯、4(2,4-二羟基苯甲酰氨基)苯甲酸、苯丙胺酸、脱氧鸟苷、鸟苷和N-乙酰酪胺;并初步评价上述化合物的抗肿瘤活性,结果表明,邻苯二甲酸二异丁酯显示细胞毒活性。To explore the antitumor active components in ethyl acetate extract of Mycale plumose sponge-derived actinomycete Saccharopolyspora sp. nov by using solvent extraction, silica gel column, PHPLC and etc, seven compounds were isolated and purified. The separation procedure was guided by a bioassay using tsFT210 cell lines. By means of physico-chemical properties and spectral analysis, their structures were determined as ; cholesterine, 1,2-benzendicarboxylic acid bis （2-methylpropyl） ester, 4- （2,4-dihydroxybenzamido） benzoic acid, phenylalanine, deoxyguanosine, guanosine, N-acetyltyramine. The antitumor activity against tsFT210 cell line of these compounds was assayed and 1,2-benzendicar-boxylic acid bis （2-methylpropyl） ester showed significant cytotoxic activity.

关 键 词：海绵共附生放线菌 羽毛山海绵 糖多孢菌 次级代谢产物 抗肿瘤活性 

分 类 号：R979.1[医药卫生—药品]