慢性心理应激小鼠胸腺免疫细胞凋亡损伤的研究  

A Study of related mechanism between immunocyte cell apoptosis and chronic psychological stress

作　　者：杨宁波[1] 吴爱勤[1] 陈文[1] 贺丹军[2] 李洁[3] 

机构地区：[1]苏州大学附属第一医院精神科,苏州215006 [2]江苏省人民医院临床心理科 [3]苏州大学附属第一医院内分泌科,苏州215006

出　　处：《中国行为医学科学》2006年第11期986-988,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的观察慢性束缚应激下小鼠胸腺病理改变及天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)的活性,探讨应激对小鼠免疫器官的损害作用及途径。方法动物随机分为空白对照组和应激组。应激组小鼠给予慢性束缚应激,每天1次,每次8h,持续2周。应激后处死小鼠,制作胸腺HE片、免疫组化Caspase-3片,在显微镜下进行免疫阳性细胞记数并显微拍照。结果应激后小鼠胸腺组织形态改变重量下降[NC组(0.1000±0.0193)gvsSS组(0.0703±0.0205)g,P<0.01],胸腺指数下降[NC组(0.0034±0.0003)vsSS组(0.0028±0.0007),P<0.05],同时应激组胸腺Caspase-3阳性细胞率增加[NC组(15.6±3.91)%vsSS组(55.2±7.12)%,P<0.01]。结论慢性心理应激能造成胸腺损伤,Caspase-3参与了应激状态下胸腺免疫细胞损伤。Objective To observe pathological changes and activity of Caspase-3 in thymus following chronic restraint stress, and inquiry into its＇damage and mechanism. Methods 16 mice were randomly divided into control and stress group （ both n = 8）. RES device were 50-ml polypropylene conical tubes, and restraint stress was imposed 8h per night. After 14 days mice were killed and thymus were taken. Paraffin section for HE and immuno-histochemistry Caspase-3 were made, and then count the positive cell and photomicrographs were taken. Results The structure and weight of thymus occur changes compare to the control group[ NC （0. 1000±0. 0193） g vs SS （0.0703 ± 0. 0205）, P 〈 0.01 ], and the thymus index of SS group decreased [ NC （0. 0034 ± 0. 0003 ） vs SS （0.0028 ± 0. 0007）, P 〈 0.05 ], meanwhile the positive cell rate of Caspase-3 in restraint group increased [NC （15.6±3.91） vs SS （55.2±7.12）, P〈0.01） ]. Conclusion The damage of thymus may result from restraint stress, Caspase-3 appears to participate the process of thymus immune cell damnification.

关 键 词：束缚应激 胸腺指数 细胞凋亡 天冬氨酸特异性半胱氨酸蛋白酶-3 

分 类 号：R392[医药卫生—免疫学]