燕麦新品种燕科一号组织培养特性及再生体系研究  

Tissue Culture Response and Regeneration System of the New Oat Variety Yanke 1

作　　者：贾利敏[1] 贾利欣 付晓峰[3] 

机构地区：[1]内蒙古农牧业科学院生物中心,内蒙古呼和浩特010031 [2]内蒙古农业技术推广站,内蒙古呼和浩特010010 [3]内蒙古农牧业科学院甜菜研究所,内蒙古呼和浩特010031

出　　处：《麦类作物学报》2006年第6期42-45,共4页Journal of Triticeae Crops

基　　金：内蒙古自治区科技厅"十五"攻关项目(200201)

摘　　要：为建立燕麦遗传转化的高效植株再生体系，选用裸燕麦新品种燕科一号的幼穗进行组织培养特性和再生体系研究。结果表明，在6种诱导培养基上愈伤组织状态和诱导率差异不大；在进一步继代培养过程中愈伤组织出现明显差异，其中培养基Y2和J结合培养效果好；愈伤组织分化产生绿色芽点和绿苗，二者分化率呈显著正相关；绿苗分化适宜的激素配比是6-BA0．5mg／L＋KT0．4mg／L＋NAA0．4mg／L，有84．90％的愈伤组织分化成苗，其中KT的作用较6-BA、NAA强；在1／2MS培养基上再生苗容易生根，生根率达到89．64％，移栽成活率91．05％；幼穗取样以0．5～1．0cm最为理想。In order to establish a high efficient plant regeneration system for gene gun transformation, the tissue culture response and regeneration system of oat were studied by using inflorescences of the new variety Yanke 1. The results showed that the callus states and inducing frequency of oat were similar in six induction medium, but there were significant difference among the callus under different medium in subculture. The culture effect was very good when medium Y2 used in combination with medium J. The differentiating frequency of green buds and green shoots exited significant and positive correlation. Based on the orthogonal test, 6-BA 0.5 mg/L＋KT 0.4 mg/L＋ NAA 0.4 mg/L were the optimal composition of medium for green shoots differentiation from callus and their frequency was 84.90%, KT had a better effect on differentiation than 6-BA and NAA. Roots inducing of regeneratied shoots were easier on 1/2 MS medium and the frequency of root inducing and transplant survival were 89.64% and 91.05%, respectively. Meanwhile the optimal length of inflorescence was 0.5～1.0 cm,

关 键 词：燕麦 燕科一号 幼穗 愈伤组织 植株再生体系 

分 类 号：S512.1[农业科学—作物学] S336