经多聚环氧化合物预处理移植静脉免疫原性的实验  

Study of immunogenecity of vascular homograft pretreated by polyepoxy compound

作　　者：张宏伟[1] 

机构地区：[1]河南省人民医院器官移植中心,郑州450003

出　　处：《中原医刊》2006年第23期1-2,共2页Central Plains Medical Journal

摘　　要：目的对经多聚环氧化合物(PC)处理的同种异体静脉的免疫原性效果的研究。方法根据处理猪静脉的不同方法分为新鲜组(n=11)、戊二醛(GA)组(n=7)和PC组(n=9);匀浆液二次免疫BALB/c小鼠,小鼠血清与该猪淋巴细胞反应,以荧光标记的兔抗鼠IgG抗体染色被反应的淋巴细胞,计算染色淋巴细胞的百分率。结果新鲜组的淋巴细胞染色率高于PC组和GA组(P<0.05),PC、GA组之间淋巴细胞染色率差异无统计学意义(P>0.05)。结论降低移植静脉的免疫原性的效果,PC和GA一样的明显有效。Objective To evaluate the immunogenecity of vascular homograft pretreated compound （PC）. Methods BALB/c mice were immunized with glytaraldehyde （GA） or PC pretreated or untreated canine vessels, using their sera collected 2-4d after the last immynization. The pig＇s peripheral lymphocytes were stained with FITC labeled anti -mous IgG antibodies against shared antigens on the vessel and lymphocytes. Percentages of stained lymphocutes were then analyzed with flow cytofluorometry. Results The pencentage of stained lymphocytes of the fresh group was significantly higher than the GA group and PC group. Conclusion PC can reduce the immunogenecity of the veinous homograft obviously.

关 键 词：多聚环氧化合物 戊二醛 同种异体移植静脉 

分 类 号：R392[医药卫生—免疫学] R654.4[医药卫生—基础医学]