竹叶眼子菜居群遗传多样性和克隆结构  

Genetic diversity and clonal structure of Potamogeton malaianus populations.

作　　者：陈媛媛[1] 栗琪[2] 吴文颖[1] 李伟[1] 

机构地区：[1]中国科学院武汉植物园水生植物生物学实验室,武汉430074 [2]中国科学院水生生物研究所,武汉430072

出　　处：《应用生态学报》2006年第11期2034-2040,共7页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(29970065);中国科学院知识创新工程项目(KSCX2-1-10);中国科学院生命科学与生物技术特别支持费资助项目.

摘　　要：采用ISSR技术对长江中游南岸豹湃湖和大冶湖不同生境中的竹叶眼子菜（Potamogeton malaianus）居群的遗传多样性及克隆结构进行了研究．结果表明，在两居群的106株个体中，利用6条ISSR引物共得到40条符合3／N标准并无连锁不平衡的清晰位点，竹叶眼子菜具有较高的遗传多样性，其多态位点百分率为75．0％，Shannon多样性指数为0．3736，两居群的遗传分化很小．竹叶眼子菜的克隆多样性很高（D=0．9917），两居群间的克隆分化很大，不具有共有的基因型．竹叶眼子菜的基株分布为游击型构型，位于湖心的大冶居群克隆距离（3．0—31．5m）明显地大于湖岸豹湃居群（2．4—6．7m）．By using inter-simple sequence repeat （ISSR） makers, this paper studied the genetic diversity and clonal structure of two Potamogeton malaianus populations growing in different habitats of two lakes in the middle reaches of Yangtze River. With six primers selected, a total of 40 fragments, of which 30 （75.0%） were polymorphic, were obtained from 106 individuals. This species showed a high genetic diversity, with Nei＇ s gene diversity of H = 0. 2471 and Shannon＇ s information index of I = 0. 3736. A low degree of population differentiation （ GSI = 0. 0861 ） was found between the two populations, and a very high clonal diversity （D =0. 9917） was detected, according to the analysis based on the polymorphic loci. No common genotypes were found among the individuals from the two populations, showing a great clonal differentiation between them. The spatial distribution pattern of P. malaianus genets showed ＇ guerilla＇ architecture, and the clonal distance of the population in lake center （3. 0 -31.5 m） was significantly larger than that in lakeshore （2. 4 - 6. 7 m）.

关 键 词：竹叶眼子菜 遗传结构 克隆多样性 克隆分化 克隆空间结构 

分 类 号：Q943[生物学—植物学]