西湖沉积物中解磷菌的分离纯化及其解磷能力  

Isolation, purification, and phosphate-solubilizing capability of phosphorous bacteria in West Lake sediment.

作　　者：李文红[1] 施积炎[1] 

机构地区：[1]浙江大学环境工程系,杭州310029

出　　处：《应用生态学报》2006年第11期2112-2116,共5页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划资助项目(2002CB410807).

摘　　要：采用有机磷固体培养基和无机磷固体培养基从沉积物中分离出具有解磷能力的菌株,通过平板划线分离纯化后得到6株磷细菌,其中2株为有机P细菌(编号为OP1、OP2),4株为无机磷细菌(编号分别为NOP1、NOP2、NOP3、NOP4).测定发现,OP1、OP2和NOP3溶磷能力较强,NOP4解磷能力较微弱,而NOP1及NOP2在分离纯化后失去了解磷能力;菌株OP1及OP2具有较强的分解有机磷卵磷脂的能力,接种OP1、OP2菌株的培养液中水溶性磷含量分别比对照增加了38·53和64·53倍;接种NOP3菌株的培养液中磷含量比对照增加了54·06倍.By using solid culture media containing organic and inorganic phosphorus, six strains of phosphorous bacteria in West Lake sediment were isolated and purified, among which, two strains coded as OP1 and OP2 could decompose lecithin, and the other four coded as NOP1 , NOP2, NOP3 and NOP4 could dissolve inorganic phosphate. OP1 OP2 and NOP3 had a stronger phosphate-solubilizing capability, followed by NOP4, while NOP1 and NOP2 lost this capability after isolation and purification. The water-soluble P concentration in the culture media inoculated with OP1 , OP2 and NOP3 increased 38.53, 64.53 and 54.06 fold, respectively, compared with the control.

关 键 词：解磷菌 磷 沉积物 确定 

分 类 号：X524[环境科学与工程—环境工程]