花生球蛋白基因克隆及其表达分析  

Cloning and Expression Analysis of an Arachin Gene from Arachis hypogaea L.

作　　者：全先庆[1] 杨辉霞[1] 单雷[2] 毕玉平[2] 

机构地区：[1]山东师范大学生命科学学院 [2]山东省农业科学院高新技术研究中心,山东济南250100

出　　处：《安徽农业科学》2006年第21期5483-5484,5490,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省自然科学基金项目(Z2002D06)

摘　　要：利用噬菌斑原位杂交技术从花生未成熟种子cDNA文库中筛选到一个花生种子贮藏蛋白基因全长cDNA,该cDNA包含一个1 886 bp的开放阅读框,可编码536个氨基酸残基,Northern分析发现,该基因在种子发育早期开始表达,在之后的各个时期表达水平均较高。A full-length cDNA of an arachin gene was cloned from a cDNA library constructed with immature peanut seeds by plaque in situ hybridization. The cDNA contained an ORF of 1 886 bp with a deduced protein of 536 amino acid residues. Results of northern blot analysis showed that the expression of the cloned gene occurred at an early stage of seed development, was increased dramatically in the following stage and retained the higher accumulation of mRNA until mature.

关 键 词：花生 球蛋白 CDNA 表达 

分 类 号：Q785[生物学—分子生物学]