抗环瓜氨酸肽抗体在类风湿性关节炎诊断中的应用  

The clinical application of Mark2 anti-cyclic citrullinated peptide determination in rheumatoid arthritis

作　　者：李小龙[1] 陶洪群[2] 张文辉[1] 郑彦博[1] 

机构地区：[1]温州医学院附属第一医院检验科,浙江温州325000 [2]温州医学院附属第二医院检验科,浙江温州325027

出　　处：《检验医学》2006年第6期599-601,共3页Laboratory Medicine

摘　　要：目的探讨M ark2(第2代)抗环瓜氨酸肽(CCP)抗体酶联免疫吸附试验(ELISA)检测类风湿性关节炎(RA)的临床应用价值。方法用M ark2和M ark1抗CCP抗体ELISA试剂盒对65例RA患者、154例其他非RA患者同时进行M ark2和M ark1抗CCP抗体检测;用速率散射比浊法检测患者血清类风湿因子(RF)浓度。结果M ark2抗CCP抗体对RA诊断的敏感性为73.8%,特异性为96.8%,诊断效率为90.0%,约登指数为0.71;而RF对RA诊断的敏感性为87.7%,特异性为81.8%,诊断效率为83.6%,约登指数为0.69;两者联合检测敏感性为96.9%,特异性为81.2%,诊断效率为84.7%,约登指数0.78。M ark1抗CCP抗体对RA诊断的阳性率为61.5%,不如第2代抗CCP抗体。结论M ark2抗CCP抗体可做为RA的辅助诊断指标。Objective To explore the diagnostic value of Mark2 anti-cyclic citrullinated peptide（anti-CCP） in patients with rheumatoid arthritis （RA）. Methods In 65 RA patients, 154 not RA patients, serum rheumatoid factor （RF） was determined by nephelometry, Markl and Mark2 anti-CCP were tested by enzyme-linked immunosorbent assay （ELISA）. Results The diagnostic sensitivity of Mark2 anti-CCP and RF in RA patients was 73.8% and 87.7%. After combined determination of anti-CCP and RF the positive rate rose to 96.9%. The diagnostic specificity of Mark2 anti-CCP and RF was 96.8% and 81.8% in RA. The diagnosis efficiency was 90.0% for Mark2 anti-CCP and 83.6% for RF. The youden＇s index was 0. 71 for Mark2 anti-CCP and 0. 69 for RF. The diagnostic specificity of combined determi- nation of Mark2 anti-CCP and RF was 81.2% , the diagnosis offieiency was 8d. 7% and the youden＇s index was 0. 78. The positive rate of Markl anti-CCP in RA was 61.5%, which were worse than Mark2. Conclusions Mark2 Anti-CCP could be regarded as a diagnostic marker in RA.

关 键 词：抗环瓜氨酸肽抗体 类风湿性关节炎 类风湿因子 

分 类 号：R446.62[医药卫生—诊断学]