培养基中碳源和氮源对甘草愈伤组织生长和黄酮类化合物合成的影响  

Effects of carbon source and nitrogen source on callus growth and flavonoid content in Glycyrrhiza uralensis

作　　者：杨世海[1] 陶静[1] 刘晓峰[2] 果德安[3] 郑俊华[3] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]吉林亚泰(集团)股份有限公司,吉林长春130031 [3]北京大学药学院,北京100083

出　　处：《中国中药杂志》2006年第22期1857-1859,共3页China Journal of Chinese Materia Medica

摘　　要：目的：研究碳源和氮源对甘草愈伤组织生物量积累和黄酮类化合物形成的影响。方法：在培养基中添加不同质量分数的蔗糖和果糖以及不同比例的NH^4＋和NO^3-，进行甘草愈伤组织的诱导和培养，并用HPLC检测愈伤组织中黄酮类化合物的含量。结果：2种碳源中，甘草愈伤组织生物量积累和黄酮类化合物合成均以果糖为碳源时高。在用2％果糖作为碳源时，培养物中5种黄酮类化合物的含量总和是3％蔗糖作为碳源的2倍。果糖作为碳源，在2％-6％时，随着质量分数的增加，培养物中5种黄酮类化合物的含量总和呈下降趋势，但其中甘草查尔酮的含量却增加5倍以上。硝态氮有利于甘草愈伤组织生长和黄酮类化合物的积累。当NH^4＋／NO3^-浓度比例为1／2时，甘草愈伤组织中5种黄酮类化合物含量的总和达最大值，为151．47μg·g^-1铵态氮抑制甘草愈伤组织生长和黄酮类化合物的形成。结论：果糖作为碳源最有利于甘草愈伤组织生物量积累和黄酮类化合物的生物合成。硝态氮有利于甘草愈伤组织生长和黄酮类化合物的积累。Objective： To study the effects of carbon source and nitrogen source on callus growth, and flavonoid content in Glycyrrhiza uralensis. Method： Induction and culture of callus were conducted in the media of different concentrations of sucrose, and fructose and ratio of NH4^＋/NO3^- ,and flavonoid content was measured by HPLC. Result： The results showed that fructose was superior to sucrose for callus growth and flavonoid formation, and the optimum concentration was 2%. The flavonoids content was 2 times higher than that of sucrose as carbon sources. In the range of 2%-6% of fructose concentration, the flavonoid content was decreased along with the concentration of fructose, but the licochalcone was increased 5-fold. The highest flavonoid content of 151. 47μg·g ^-1 was obtained when the ratio of NH4^＋/NO3^- in the medium was 1/2. NH^4＋ inhibited the callus growth and flavonoid formation of G. uralensis. Conclusion： Fructose as carbon source was superior to sucrose for callus growth and flavonoid formation, and NO3^- was favorable to the callus growth and flavonoids accumulation.

关 键 词：甘草 愈伤组织 碳源 氮源 黄酮类化合物 

分 类 号：S567.71[农业科学—中草药栽培]