扶正健肝方对白蛋白所致免疫性肝纤维化大鼠的影响  

Effect of Fuzheng Jiangan formula on liver fibrosis induced by albumin in rats

作　　者：黄象安[1] 孙利红[1] 宋崇顺[2] 崔宁[1] 陈燕力[1] 徐洪雁[1] 任颖[2] 

机构地区：[1]北京中医药大学东方医院感染性疾病科,北京100078 [2]北京中医药大学东直门医院中药药理学实验室,北京100700

出　　处：《中国中药杂志》2006年第22期1890-1893,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察扶正健肝方对白蛋白诱导的大鼠肝纤维化形成的影响。方法:采用人血白蛋白(HSA)诱导大鼠肝纤维化并随机分为扶正健肝方高、低剂量组(生药39.4,9.85 g.kg-1),秋水仙碱组(0.000 1 g.kg-1),模型组,对照组5组。观察血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性、总蛋白(TP),白蛋白(A lb)含量球蛋白和白蛋白比值。放射免疫分析法测定层连蛋白(LN)、透明质酸(HA)和Ⅳ型胶原(IV-C)的含量,化学法测定肝脏羟脯氨酸(Hyp)的含量。光镜观察肝细胞结构和肝纤维化程度。结果:扶正健肝方能明显降低人血白蛋白诱导的肝纤维化大鼠ALT,AST活性(P<0.05,P<0.01),提高A lb,TP含量及A/G比值(P<0.05,P<0.01),降低血清LN,HA IV-C含量(P<0.05,P<0.01)。病理学观察结果,扶正健肝方能使大鼠胶原纤维沉积明显减轻,假小叶结构明显减少。并明显降低纤维化计分(P<0.05,P<0.01)。结论:扶正健肝方可抑制人血白蛋白诱导的大鼠肝纤维化形成和发展。Objective： To observe the effect of Fuzheng Jiangan formula（FZJGF） on liver fibrosis using immune induced liver fibrosis rat model. Method： The rat models with immunity liver fibrosis were induced by the human serum albumin. Rats were treated with normal saline, FZJGF （9. 85,39. 4 g · kg^-1, two dosage groups） and Colchicine （ 0. 000 1 g · kg^-1 ）. The activities of ALT, AST, contents of ALB and TP, and A/G, The contents of Laminin （LN）, Hyaluronic acid （HA） and collagen type Ⅳ（Ⅳ-C） in rat serum were measured by radioimmunoassay method. The level of hydroxyproline （Hyp） in liver was detected by chemistry method. The pathological changes of liver tissue were observed by HE and Von-Gieson staining. Result： FZJGF could significant decrease the serum activities of ALT and AST, and increase the levels of TP,Alb and ratio A/G. The levels of LN, HA and Ⅳ-C were decreased significantly after the treatment using FZJGF. The pathological improvements were observed. FZJGF could markedly alleviate the deposition of collageneous fiber, and reduce the liver pseudoluboli and the fibrosis scores in the liver tissue compared with model group. Conclusion： FZJGF can inhibit formation and development of rat hepatic fibrosis induced by the human serum albumin.

关 键 词：扶正健肝方 肝纤维化 免疫 人血白蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]