百合无症病毒的RT-PCR检测  

Detection of lily symptomless virus by RT-PCR

作　　者：马书智[1] 陈臻[1] 徐秉良[1] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2006年第5期60-62,共3页Journal of Gansu Agricultural University

基　　金：由国家科技部项目(2001EA86005);兰州市科委项目(03-1-39)共同资助.

摘　　要：以百合病株为材料,针对百合无症病毒(LSV)的CP基因序列,设计合成了一对特异引物,并通过RT-PCR技术扩增出与预期大小相一致的870 bp片段,而对照无任何产物,应用该方法对田间百合中的LSV的带毒情况进行检测,检出率达80%,检测灵敏度高,专一性强,为百合的病毒检测提供了一种分子生物学检测方法.Lily symptomless virus （LSV）was detected in several lily cultivars by the reverse transcription and polymerase chain reaction（RT-PCR）. A pair of primers were designed and synthesized based on the nueleotide sequenee of the CP gene of LSV genome. By RT-PCR,a 870bp DNA fragment was amplified from LSV-RNA,while no fragment was obtained from the control. The results showed RT-PCR was a sensitive,specific and reliable method for the detection of LSV. LSV was detected in 8 out of 10 lily samples in the field,and the infection rate was 80%.

关 键 词：百合无症病毒 RT-PCR 检测 

分 类 号：Q789[生物学—分子生物学]