川芎嗪对烧伤延迟复苏大鼠血管通透性的调控效应  

Regulation effect of Ligustrazine on vasopermeability of rats with delayed resuscitation due to burn shock

作　　者：张春海 傅廷友 蔡莉 王光毅[2] 唐洪泰[2] 

机构地区：[1]解放军四一医院创伤外科,山东省青岛市266100 [2]解放军第二军医大学长海医院烧伤中心,上海市200433

出　　处：《中国临床康复》2006年第43期120-122,共3页Chinese Journal of Clinical Rehabilitation

基　　金：军队"十五"指令性项目资金(01L055)~~

摘　　要：目的:观察川芎嗪在烧伤休克延迟复苏大鼠中的抗渗作用及对肿瘤坏死因子α、白细胞介素1β、白细胞介素10的调控。方法:实验于2003-07/2005-05在上海长海医院烧伤中心实验室完成。取36只成年健康SD大鼠,体质量200～220g,随机数字表示法分为假烫组、烫伤组、治疗组,每组12只。①烫伤组大鼠采用恒温水烫仪以100℃水烫背部13s,造成大鼠30%总体表面积Ⅲ度烧伤。假烫组大鼠以38℃水模拟烫伤过程;治疗组伤后半小时腹腔注射川芎嗪(80mg/kg);烫伤组及治疗组均于伤后6h腹腔注射乳酸林格液进行延迟复苏。②各组半数大鼠于伤后24h行腹主静脉取血处死,保留静脉血,留取肺脏、心脏及肾脏标本检测含水量;另半数大鼠于处死前1h颈外静脉注入1%伊文思蓝,留取肺脏、心脏及肾脏标本检测脏器血管通透性。③采用酶联免疫吸附法分别测定伤后24h后血浆中肿瘤坏死因子α、白细胞介素1β、白细胞介素10的浓度水平。结果:纳入大鼠36只,均进入结果分析。①烫伤后24h,烫伤组大鼠的肺脏、心脏及肾脏的含水量高于假烫组及治疗组[烫伤组(82.24±2.15),(79.69±2.36),(79.31±1.65)%,假烫组(76.35±1.88),(74.16±1.59),(73.29±2.38)%,P<0.01,治疗组(79.71±1.94),(76.51±1.82),(75.82±1.69)%,P<0.05];假烫组与治疗组大鼠的肺脏、心脏及肾脏组织含水量差异均有显著性意义[(76.35±1.88),(74.16±1.59),(73.29±2.38)%,(79.71±1.94),(76.51±1.82),(75.82±1.69)%,P<0.05]。②烫伤组大鼠脏器的血管通透性显著高于假烫组及治疗组[烫伤组(437.19±39.23),(313.69±46.54),(346.51±32.27)mg/L,假烫组(49.46±10.16),(62.86±19.38),(59.14±23.24)mg/L,治疗组(293.18±18.96),(185.72±25.49),(201.38±21.98)mg/L,P<0.01]。③烫伤组大鼠血清中肿瘤坏死因子α、白细胞介素1β、白细胞介素10的浓度与假烫组差异非常显著[(612.30±75.03),(208.45±49.04),(221.39±33.14)ng/L,(32.68±9.33),(15.87±7.46),(6.99±2.84)ngAIM： To investigate the anti-permeability effect of Ligustrazine as well as its regulation effects on tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β） and IL-10 in rats with delayed fluid resuscitation after severe burn. METHODS： The experiment was conducted in the Central Laboratory of Burns Center of Changhai Hospital between July 2003 and May 2005. Thirty-six healthy adult SD rats weighing 200-220 g were adopted and divided into sham-burn group, burn group and treatment group randomly with twelve rats in each group.（1）The dorsal surface areas on skin of rats in the burn group were immersed into 100℃ water for 13 seconds by the constant temperature template device, and full-thickness dermal burns comprising 30% total body surface area were obtained. Rats in the sham-burn group were immersed into 38℃ water for simulation. Ligustrazine was administered intraperitoneally at 80 mg/kg in rats of the treatment group at 30 minutes post-burn. Rats in the burn and treatment groups were resuscitated by intraperitaneal injection of Ringer lactate solution at 6 hours post-burn.（2）Half of rats in each group were exsanguinated by ventrocardinal vein puncture at 24 hours after burnt or sham-burn, and venous blood were reserved, then the samples of lungs, hearts and kidneys were remained to detect water contents; The other half of rats were administered with 1% Evans blue from external jugular vein at 1 hour before execution, and samples of lungs, hearts and kidneys were remained to detect vasopermeability. （3）The levels of TNF-α, IL-1β and IL-10 in sera were assayed by ELISA respectively at 24 hours after burn. RESULTS： Thirty-six enrolled rats were involved in the analysis of results. （1）The water contents of lungs, hearts and kidneys in rats of the burn group at 24 hours after burn were significantly higher than those in the sham-burn and treatment groups [burn group： （82.24±2.15）, （79.69±2.36）, （79.31±1.65）%; sham-burn group： （76.35±1.88）, （74.16�

关 键 词：烧伤 血管通透性 细胞因子类 

分 类 号：R826.5[医药卫生—临床医学]