猪链球菌2型广西GX01株Cps2J基因克隆与序列分析  

Sequencing and Analysis of Cps2J of Streptococcus suis Serotype 2 GX01 Strain

作　　者：熊毅 覃芳芸[2] 白昀[2] 朱伟[2] 郭建刚 李华明 陆立权 邹联斌 刘棋 

机构地区：[1]广西兽医防疫检疫站,广西南宁530001 [2]广西大学动物科技学院,广西南宁530005

出　　处：《动物医学进展》2006年第11期58-60,共3页Progress In Veterinary Medicine

基　　金：广西科技厅科技攻关与新产品试制项目(0632002-1)

摘　　要：将分离到的广西猪链球菌2型，用针对猪链球菌2型Cps2J序列的引物Cps1／Cps2进行扩增，得到与预期相符的675bp的片段，将PCR产物回收连接转化后测序，得到了675bp的核苷酸序列，经同源性分析表明，GX01株Cps2J序列与猪链球菌2型国内外的毒株核苷酸同源性在98．7％～100％之间，具有高度的同源性；与猪链球1型的同源性只有66．7％。The Cps2J of GX01 strain which isolated from pigs of Guangxi was amplified by Cps1/Cps2, then the product of PCR was sequenced and analyzed. The result showed that the Cps2J sequenee homologies of GX01 and other of Streptococcus suis type 2,whieh published in GenBank, were 98. 7% to 100%, the homology between GX01 and Streptococcus suis type 1（AF155804） was 66.7%.

关 键 词：猪链球菌2型 Cps2J 序列分析 

分 类 号：S852.611[农业科学—基础兽医学]