SIV与PRRSV、CSFV、PCV-2和PRV交叉感染的检测  

Detection of Co-infection with SIV and PRRSV,CSFV,PCV-2 and PRV

作　　者：蒙雪琼[1] 刘棋 陈义祥 黄夏 赵国明 黄胜斌 胡丽萍 陈芳芳 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西兽医防疫检疫站,广西南宁530001

出　　处：《动物医学进展》2006年第11期84-86,共3页Progress In Veterinary Medicine

摘　　要：采用RT-PCR方法对37份疑似猪流感病毒(SIV)感染病料进行了病原学检测。同时,还运用PCR和RT-PCR方法对SIV阳性病料进行了猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)、猪圆环病毒2型(PCV-2)和猪伪狂犬病毒(PRV)目的基因片段的扩增。结果,扩增出了SIV特异目的基因片段,且4份病料都为混合感染,感染状况分别为SIV/PRRSV/PRV,SIV/CSFV/PRRSV三重感染,SIV/PCV-2和SIV/PRRSV二重感染。In an effort to know the co-infection with SIV and other viruses, detection of swine influenza virus （SIV） by reverse-transcription polymerase chain reaction （RT-PCR） was performed on some suspect samples collected from different places in March 2005. In addition, those suspect field eases were also detected for the presence of Porcine reproductive and respiratory syndrome virus （PRRSV）, Classical swine fever virus （CSFV）, porcine eireovirus type-2 （PCV-2） and Pseudorabies virus （PRV） by means of RT- PCR and PCR techniques. SIV/PRRSV/PRV, SIV/CSFV/PRRSV, SIV/PCV-2 and SIV/PRRSV co-infections were identified in the 4 samples from Nanning, Liuzhou, Yulin cities and Hainan province, respectively. The results from this study indicated that co-infection with SIV and PRRSV,CSFV, PCV-2 and/or PRV were universal, and may increase the possibility of epidemic occurrence and the severity of the diseases.

关 键 词：猪流感病毒 猪繁殖与呼吸综合征病毒 猪瘟病毒 猪圆环病毒2型 猪伪狂犬病病毒 混合感染 

分 类 号：S852.659.5[农业科学—基础兽医学]