抗氧化剂硫辛酸对实验性ALI/ARDS大鼠的保护作用研究  

Protective Efffect of Antioxidant Lipoic Acid on Experimental ARDS/ALI Rats

作　　者：顾红军[1] 孙沁莹[1] 李强[1] 

机构地区：[1]上海长海医院呼吸科,上海市200433

出　　处：《中国药房》2006年第22期1702-1704,共3页China Pharmacy

摘　　要：目的研究代谢性抗氧化剂硫辛酸(LA)对急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)模型大鼠的保护作用,为ALI/ARDS药物治疗提供新的途径。方法将ALI/ARDS大鼠分为正常对照组(生理盐水(NS)组)、ALI/ARDS模型组(脂多糖(LPS)组)、LA干预组(LA组)和谷胱甘肽干预组(GSH组),LPS注射30min后分别尾静脉注射LA和GSH。并于注射LPS和NS1、2、4、6h后动态观察血清α-干扰素(TNF-α)水平,6h处死大鼠,观察光镜和电镜下肺组织的病理改变,测定动脉血氧分压(PaO2)、血清脂过氧化物(LPO)水平、肺湿干比(W/D)、肺泡灌洗液(BALF)中蛋白浓度和TNF-α水平。结果LA组光镜和电镜下病理表现较LPS组均有好转;与LPS组比较,LA组和GSH组PaO2水平明显升高(P<0·01),LPO、W/D、BALF中蛋白浓度明显下降(P<0·01);LA和GSH能明显抑制BALF中TNF-α水平;血清中TNF-α水平进行性下降,第1、4、6h时有统计学意义(P<0·01)。结论LA对LPS诱发大鼠ALI/ARDS有保护作用。OBJECTIVE： To study the protection effect of lipoic acid on acute lung injury/ acute respiratory distress syndrome （ALI/ARDS） model rats and to provide new therapy for ALI/ARDS. METHODS： Experimental ALI/ARDS mode rats were divided into control group （NS group）; ALI/ARDS group （LPS group）; LA group and GSH group. LA and GSH were administered in the vena caudalis of rats at 30min after administration of LPS. Serum TNF- α level was measured at 1, 2, 4 and 6h after administration of LPS or NS. The rats were put into death at 6h. The histopathological change of lung tissues were observed under light microscopy and electron microscopy, and PaO2, LPO, W/ D and the protein concentration and TNF-α level in Balf were determined. RESULTS; Histopathologically, LA group has a better improvement than LPS group either under light microscope and electron microscope. PaO2 level were significantly higher （P〈0.01） while the protein concentration in LPO, W/ D and BALF were significantly lower （P〈0.01） in LA group and GSH group as compared with LPS group. TNF- α level in Balf was significantly inhibited in LA group and GSH group. The level of TNF-α in serum decreased progressively, and there were significances at 1, 4 and 6h （P 〈 0.01）. CONCLUSION ： LA has protective effects on LPS-induced acute lung injury model rats.

关 键 词：急性肺损伤 急性呼吸窘迫综合征 硫辛酸 大鼠 Α-干扰素 

分 类 号：R965[医药卫生—药理学]