三种常用抗生素对苎麻种子发芽及子叶再生的影响  

Effect of Three Antibiotics on Seed Germination and Plant Regeneration from Cotyledons of Ramie [Boehmeria nivea (L.) Gaud]

作　　者：汪波[1] 彭定祥[1] 孙珍夏[1] 张娜[1] 高世梅[1] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070

出　　处：《分子植物育种》2006年第6期895-899,900,共6页Molecular Plant Breeding

基　　金：国家863项目麻类作物高效育种技术及优质高产多抗新品种选育（2001AA241121）资助.

摘　　要：本研究测试了卡拉霉素、羧苄青霉素、头孢霉素对于3个不同基因型苎麻子叶再生的影响,还测试了卡拉霉素对于苎麻种子发芽及生长的影响,结果表明:苎麻子叶对于卡拉霉素有着较高的敏感性,20～25mg/L的卡拉霉素就能完全抑制苎麻子叶再生;羧苄青霉素能显著抑制苎麻子叶再生;浓度在500mg/L以下的头孢霉素对苎麻子叶再生没有明显影响。此外,苎麻种子对于卡拉霉素也较敏感,100mg/L卡拉霉素处理30d能使苎麻种子实生苗全部黄化(白化)死亡。The effect of kanamycin, carbenicilin, cefotaxine on shoot regeneration from cotyledon explants of ramie and the effect of kanamycin on seed germination and growth were evaluated. The results showed that cotyledons of ramie were highly sensitive to kanamycin and no shoots could be regenerated from cotyledon explants on medium supplemented on 20 to 25mg/L kanamycin. Carbenicilin significantly inhibited plant regeneration from cotyledon explants, while cefotaxine under 500mg/L has no significant effect on plant regeneration from cotyledon explants of ramie. Furthermore, ramie seed was sensitive to kanamycin and seed cultured on medium supplemented with 100mg/L kanamycin for 30 days became completely yellow or white.

关 键 词：抗生素 器官发生 遗传转化 苎麻 子叶 

分 类 号：S563.1[农业科学—作物学]