黄姜中薯蓣皂甙元的提取及定性定量分析  

Qualitative and quantitative analysis of diosgenin distilled from Dioscorea zingiberensis

作　　者：任凤莲[1] 刘咏[1] 禹文峰[1] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083

出　　处：《广西植物》2006年第6期684-686,642,共4页Guihaia

摘　　要：对黄姜中提取的薯蓣皂甙元进行红外和紫外可见波谱扫描，与标准品谱图对比分析，可知提取出的皂甙元与薯蓣皂甙元标准品有相同的结构。用分光光度法测定黄姜中薯蓣皂甙元的含量，以香荚兰醛-高氯酸-冰醋酸的加入顺序，在80℃水浴中加热15min，显色30min后，在542nm处测定吸收值能达到最佳值，最后得出皂甙元含量为2．27％，RSD为1．1％，回收率在97．2％～98．8％之间。UV-V（ultraviolet-visible）and infrared spectrum analysis of diosgenin were fulfilled and it could be eoneluded that the diosgenin from Dioscorea zingiberensis has the same strueture as standard＇s. The eontent of diosgenin was determined by speetrophotometer method. Vanillin, perehlorie aeid and aeetie aeid reaeted with diosgenin in sequenee, then the solution was heated by hot water at 80 ℃ for 15 min and plaeed for 30 min. At 542 nm,the content of diosgenin in D. zingiberensis was measured to be 2.27% ,RSD= 1.1% ,and the reeovery is 97.2%-98.8%.

关 键 词：薯蓣皂甙元 光谱 分光光度法 

分 类 号：Q946[生物学—植物学]