玄参中环烯醚萜Epibueropyridinium A预防D-半乳糖性白内障的实验研究  

Prevention of D-galactose-induced cataract in rats by Epibueropyridinium A extracted from Scrophularia ningpoensis

作　　者：黄才国[1] 魏善建[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2006年第11期1204-1206,共3页Academic Journal of Second Military Medical University

基　　金：上海-SK研究发展基金(2004004-t)~~

摘　　要：目的:探讨玄参中环烯醚萜Epibueropyridinium A对D-半乳糖性白内障形成的干预作用。方法:大鼠随机分为正常对照组、模型组、样品高、低剂量组和阳性对照组,每组10只。模型组腹腔注射D-半乳糖诱导大鼠白内障模型;样品高、低剂量组在注射D-半乳糖同时分别给予20、10 mg/kg环烯醚萜Epibueropyridinium A灌胃;正常对照组仅仅灌胃生理盐水;晶体混浊度和山梨醇含量的变化阳性对照为依帕司他(10 mg/kg),SOD活性和丙二醛含量变化的阳性对照为VitE(30 mg/kg)。3、6周后用裂隙灯观察晶体混浊程度,处死动物后检测晶体中SOD的活性,丙二醛以及山梨醇的含量。结果:样品高、低剂量组大鼠白内障的晶体混浊度明显低于模型组,样品高剂量组低于低剂量组(P<0.01)。晶体中SOD活性(Nu.mg-1)由模型组的32.1±11.2分别升高到样品低剂量组的65.9±16.7和样品高剂量组的83.2±15.6(P<0.01);丙二醛含量(nmol.mg-1)由模型组的26.72±5.36分别降到样品低剂量组的18.34±4.29和样品高剂量组的15.66±5.47(P<0.01);山梨醇含量(μmol.g-1)在第3周时由模型组的24.33±2.57分别降到样品低剂量组的15.46±2.07和样品高剂量组的11.45±1.39(P<0.01),在第6周时由模型组的20.04±1.59分别降到样品低剂量组的15.82±1.42和样品高剂量组的13.22±1.37(P<0.05或0.01)。结论:玄参中环烯醚萜Epibueropyridinium A对大鼠糖性白内障形成有一定的预防作用。Objective：To investigate the effect of Epibueropyridinium A （EA）, extracted from Scrophularia ningpoensis, in prevention of D-galactose-induced cataract in rats. Methods： SD rats were randomly divided into 5 groups （n： 10）, namely, the normal control, cataract model, 20 mg/kg EA, 10 mg/kg EA, and positive control groups. The cataract model was induced by intraabdominal administration of D galactose into rats. Rats of the 2 EA groups received corresponding amount of EA and those in the positive control group received epalrestat （10 mg/kg） or VitE （30 mg/kg）. The changes of the lens were examined with silt lamp microscope at defined time points. The activities of superoxide dismutase（SOD） and the content of malonaldehyde （MDA） and sorbitol were determined in the lens 3 and 6 weeks later. Results： EA obviously improved the lens opacification in the 2 EA groups compared to cataract model group, and the improvement in high EA group was more obvious than that in the low EA group（P〈0. 01）. The activities of SOD raised from （32.1±11.2） Nu . mg l in cataract model group to （65.9±16.7） Nu .mg6-11 in low EA group and （83.2±15.6） Nu . mg^-1 in high EA group（P〈0.01）. The content of MDA reduced from （26.724-5.36） nmol.mg^ 1 to （18. 34±4. 29） nmol.mg^-1 in low EA group and （15. 664±5. 47） nmol . mg^-1 in high EA group（P〈0.01）. The content of sorbitol in lens reduced from （24. 334±2.57）μmol.g^-1 to （15. 46±2. 07）μmol.g^-1 in the low EA group and （11. 45±1.39）μmol.g^-1 in the high EA group after 3 weeks（P〈0.01） ; and after 6 weeks,it reduced from （20.04±1.59） μmol . g^-1 to （15.82±1. 42）μmol . g^-1 and （13. 22±1. 37）μmol.g^-1,respectively（P〈0.05 or 0.01）. Conclusion： Epibueropyridinium A has preventive effect on development of sugar cataract in experimental models.

关 键 词：白内障 Epibueropyridinium A 超氧化物歧化酶 丙二醛 山梨醇 

分 类 号：R286.81[医药卫生—中药学]