高胆固醇对兔oddi括约肌BK通道β1亚基蛋白表达的影响  

The influence on the BK channel β1 subunit expression of SO caused by high cholesterol in rabbits

作　　者：杜滂[1] 魏经国[1] 崔光彬[1] 王亚蓉[1] 张孝勇[1] 马克军[1] 

机构地区：[1]第四军医大学唐都医院放射科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2006年第6期710-712,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30470787)

摘　　要：目的:研究高胆固醇作用下兔oddi括约肌(SO)钙依赖性钾通道β1亚基蛋白水平的变化。方法:制备鼠抗兔SO细胞钙依赖性钾通道β1亚基抗血清,进行免疫组化染色,观察高胆固醇对oddi括约肌钙依赖性钾通道β1亚基蛋白表达的影响。结果:免疫组化染色显示,高胆固醇组SO细胞钙依赖性钾通道β1亚基的蛋白表达降低,半定量分析显示,高胆固醇兔oddi括约肌BKca通道β1亚基蛋白表达水平与对照组比较有统计学意义。结论:高胆固醇可下调兔oddi括约肌BKca通道β1亚基蛋白表达的水平,从而影响BKca通道的功能。[ Abstract] AIM： To investigate the effects on protein expression of big-conductance Ca2＋-sensitive K＋ channel （BKca） /31 subunit caused by high cholesterol in Rabbit Od- di＇s sphincter （SO） cells, METHODS： The rat-anti-rabbit polyclonal antiserum against/31 subunits of BKca channel of SO cell was prepared. And the protein expression of BKca channel /31 subunit of SO tissue was detected by semi- quantitative immunohistochemical staining. RESULTS： The protein expression of BKca channels/31 subunit of SO tissue in HC group was reduced, and there＇s statistically signifi- cant difference between the HC group and the control group. CONCLUSION： High cholesterol can reduce the protein expression of BK Channel＇s /31 subunit in Rabbits＇ SO which suggests high cholesterol can affect the function of BKca channel.

关 键 词：胆固醇 钙依赖性钾通道 Β1亚基 ODDI括约肌 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]