需钙蛋白酶抑制剂I对LPS攻击的RAW264.7细胞中iκBα表达和细胞因子分泌的影响  

Effect of calpain inhibitor I on iκBα expression and cytokine secretion in RAW264.7 cells attacked with LPS

作　　者：程晓刚[1] 粟永萍[1] 罗成基[1] 刘晓宏[1] 

机构地区：[1]第三军医大学预防医学系全军复合伤研究所国家创伤烧伤复合伤重点实验室复合伤实验分室

出　　处：《细胞与分子免疫学杂志》2006年第6期720-722,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重点基础研究发展规划(973)资助(G1999054201)

摘　　要：目的:探讨需钙蛋白酶抑制剂I(CI-I)对LPS攻击RAW264.7细胞后iκBα表达和细胞因子分泌的影响。方法:用CI-I预处理RAW264.7细胞1h后,再用LPS攻击,分别用Westernblot和ELISA检测RAW264.7细胞iκBα蛋白表达和TNF-α、IL-6的分泌。结果:CI-I预处理RAW264.7细胞后,可抑制LPS导致的iκBα表达降低。LPS攻击RAW264.7细胞后4h和8h,TNF-α和IL-6的分泌均增加,CI-I和地塞米松(DEX)可抑制该效应,并具有协同抑制作用。结论:CI-I和DEX可抑制LPS攻击后RAW264.7细胞中iκBα表达和炎性细胞因子分泌,从而有助于减轻细胞损伤。AIM： To explore the effect of calpain inhibitor I（CI-I） on iκBα expression and cytokine secretion in RAW264.7 cells after LPS attack. METHODS： RAW264.7 cells were pretreated with CI-I for 1 h and attacked with LPS. The iκBα expression and TNF-α and IL-6 secretion were detected by Western blot and ELISA, respectively. RESULTS： CI-I inhibited the decrease of iκBα expression in RAW264. ？ cells treated with LPS. The secretion of both TNF-α and IL-6 secretion was notably increased after RAW264.7 cells were treated with LPS for 4 h or 8 h. CI-I and dexamethasone（DEX） inhibited these effects, and the combination of DEX and CI-I had synergistic effect. CONCLUSION： CI-I and DEX can inhibit the decrease of iκBα expression and prevent TNF-α and IL-6 secretion in RAW264. 7 cells attacked with LPS, which contributes to the alleviation of cellular injury.

关 键 词：需钙蛋白酶抑制剂I IΚBΑ 地寨米松 TNF—α IL-6 

分 类 号：R392.11[医药卫生—免疫学]