尿中IL-18水平与狼疮性肾炎肾脏病理改变参数关系的评估  

Evaluation of the relationship between IL-18 levels in urine and paramenters of renal pathological changes in patients with lupus nephritis

作　　者：刘华锋[1] 许勇芝[1] 姚翠微[1] 陈婷[1] 洪涛[1] 梁东[1] 陈孝文[1] 

机构地区：[1]广东医学院附属医院肾病研究所,广东湛江524001

出　　处：《细胞与分子免疫学杂志》2006年第6期777-778,780,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东省教育厅资助项目(Z02043)

摘　　要：目的:探讨尿中IL-18的水平与狼疮性肾炎(LN)肾脏病理改变参数之间的关系。方法:应用ELISA法测定19例正常人和55例LN患者晨间自由尿及24h尿中IL-18的水平,应用直线相关分析法分析晨间自由尿及24h尿中IL-18的水平与LN肾脏病理改变的参数,即活动指数(AI)和慢性指数(CI)的相关性。结果:LN组晨间自由尿及24h中IL-18的水平均较正常对照组显著增高。两组晨尿中IL-18的水平分别为:(247.1±317.5)ng/L和(20.3±14.5)ng/L(P<0.001);24h尿中IL-18的水平分别为(192.1±170.1)ng/d和(21.0±3.8)ng/d(P<0.001),晨间自由尿及24h尿中IL-18的水平,均与LN病理改变参数AI呈密切正相关(晨尿:r=0.602,P<0.001;24h尿:r=0.461,P<0.005);但二者均与病理改变参数CI无相关性(P>0.05)。按AI值将LN患者分为高、中、低3个组,各组尿中IL-18的水平差异显著。晨尿中分别为:(69.2±82.7)ng/L、(193.5±106.1)ng/L和(580.7±453.1)ng/L(P<0.001);24h尿中分别为(103.5±141.4)ng/d、(188.8±124.0)ng/d和(333.1±183.2)ng/d。结论:检测晨间自由尿中IL-18的水平很简便,可作为评估LN肾脏病理活动程度的一种好方法。AIM： To evaluate the relationship between IL-18 levels in urine and paramenters of renal pathological changes in patients with lupus nephritis （LN）. METHODS： IL-18 levels in morning free urine and 24-hour＇s urine in 19 normal persons and 55 patients with LN were measured by ELISA. The correlation between IL-18 levels and parameters of renal pathological changes, namely activity index （AI） and chronicity index （ CI）, were analyzed by liner correlation analysis method. RESULTS. IL-18 levels in morning free urine and 24-hour＇s urine in LN group were elevated significantly compared with control group. In both groups IL- 18 levels in morning free urine were （247.1 ± 317.5） ng/L and （20.3 ± 14.5） ng/L, respectively, P 〈 0.001 ; those in 24-hour＇s urine were （192.1 ±170.1） ng/d and （21.0± 3.8） ng/d, respectively, （ P 〈0. 001 ）. There was close positive correlation between IL-18 levels in morning free urine and 24-hour＇s urine and LN patient＇s AI （for morning free urine： r=0.602, P〈0.001; for 24-hour＇s urine： r= 0.461, P〈0.005） but there was no correlation between IL- 18 levels in morning urine and 24-hour＇s urine and CI （ P 〉 0.05 ）. Patients with LN were divided into three groups （high, moderate and low） accordig to AI value. There was distinct difference of IL-18 levels in urine among the three groups： IL-18 levels in morning urine were （69.2 ± 82.7） ng/L, （ 193.5 ± 106.1 ） ng/L and （580.7 ±453.1 ） ng/L, respectively, （ P 〈 0. 001 ） ; those in 24-hour＇ s urine were （ 103.5 ± 141.4） ng/d, （ 188.8 ± 124.0） ng/d and （333.1 ±183.2） ng/d, respectively. CONCLUSION： It is very simple and convenient to detect IL-18 levels in morning free urine , so it is a good method for evaluating renal pathological activity of LN.

关 键 词：IL-18 尿液 狼疮性肾炎 活动指数 慢性指数 

分 类 号：R692.3[医药卫生—泌尿科学]