2-甲氧雌二醇抑制子宫蜕膜化基质细胞VEGF表达的研究  

2-Methoxyestradiol Inhibits Expression of VEGF on Decidual Endometrial Stromal Cells

作　　者：王蕾[1] 朱焰[2] 蒋秀蓉[2] 张倩[3] 夏鹏[3] 房素萍[4] 孙祖越[2] 曹霖[2] 

机构地区：[1]复旦大学医学院,上海200032 [2]上海市计划生育科学研究所药理毒理学研究室中国生育调节药物毒理检测中心,上海200032 [3]复旦大学药学院,上海200032 [4]上海市计划生育科学研究所附属上海博康生殖医学医院,上海200032

出　　处：《生殖与避孕》2006年第11期659-664,671,共7页Reproduction and Contraception

基　　金：国家自然科学基金资助(30500631)项目

摘　　要：目的:研究2-甲氧雌二醇对子宫蜕膜化基质细胞血管内皮生长因子(VEGF)表达的影响。方法:采用醋酸甲基孕酮及双丁酰环磷酸腺苷诱导子宫内膜基质细胞蜕膜化。诱导成功后,分别加入3个浓度的2-甲氧雌二醇,给药48 h后收集细胞上清液,采用ELISA法测定VEGF分泌情况;同时抽提细胞RNA,Real-Time RT-PCR检测VEGF mRNA水平;免疫细胞化学法测定细胞雌激素受体(ER)的表达。结果:ELISA检测表明1μmol/L、10μmol/L、50μmol/L浓度的2-ME2均可降低蜕膜化基质细胞分泌VEGF的水平;Real-Time RT-PCR同样支持上述结果;免疫细胞化学结果表明,2-ME2对蜕膜化基质细胞ER表达无影响。结论:2-甲氧雌二醇可降低蜕膜化基质细胞VEGF的表达,但与ER表达不相关。Objective： To investigate the effect of 2-methoxyestradiol on the expression of vascular endothelial growth factor （VEGF） during decidualization by endometrial stromal cells （ESCs） in vitro. Methods： ESCs were separated by enzymic digestion and filtration, and were cultured with DMEM/F12 in 10% fetal calf serum （FCS） and treated with medroxyprogesterone acetate （MPA） and dibutyryl-cyclic adenosine monophosphate （db-cAMP） to induce decidualization in vitro. After administration of 2-methoxyestradiol for 48 h, the levels of VEGF were measured by enzyme-linked immunosorbent assay （ELISA）; the expression of VEGF mRNA by decidualized cells was analyzed by Real-Time RT-PCR and the expressions of ER was measured by immunocytochemical methods. Results： In treated cells, PRL production significantly increased due to treatment with both db-cAMP and MPA. VEGF production was also significantly greater in decidualized cells than in control cells, and its level can be reduced by three concentrates of 2-methoxyestradiol. Similar findings were obtained in Real-Time RT-PCR. Immunocytochemical studies demonstrated that 2-methoxyestradiol did not influence the staining intensity of ER. Conclusion： 2-Methoxyestradiol may reduce VEGF production in this in vitro study and its action is not transmitted throughout ER signaling pathways.

关 键 词：2-甲氧雌二醇 血管生成 子宫内膜蜕膜化 血管内皮生长因子(VEGF) 

分 类 号：R96[医药卫生—药理学]