不同频率低强度脉冲电磁场对表皮干细胞增殖的影响  

The effects of a low intensity pulsed electromagnetic field on the proliferation of epidermal stem cells

作　　者：张鸣生[1] 何斌[2] 朱洪翔[2] 吴博[2] 庞文君[2] 李东风[2] 林秋雄[2] 刘旋安[2] 徐达传[1] 

机构地区：[1]南方医科大学临床解剖研究所,广州510515 [2]广东省人民医院

出　　处：《中华物理医学与康复杂志》2006年第11期726-729,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：广东省自然科学基金资助项目(No.5001279);广东省科技攻关资助项目(No.2005B34001007)

摘　　要：目的观察不同频率的低强度脉冲电磁场(LIPEMF)对皮肤表皮干细胞(ESC)增殖作用的影响。方法取人包皮皮片体外分离培养的第2代ESC,分为对照组与LIPEMF组,LIPEMF组又根据不同刺激频率分为1,10和50 Hz组。对各组细胞进行细胞克隆计数,计算克隆形成率(CFE),采用MTT法检测细胞增殖水平。结果不同频率LIPEMF的刺激均能促进ESC增殖,频率不同,促增殖效应不一．50 Hz组作用效果最为明显,与对照组比较,生长曲线明显上移。ESCβ1整合素和角蛋白19染色结果呈阳性。不同频率的LIPEMF组ESC的CFE明显高于对照组(P<0．01),50 Hz组的细胞CFE明显高于1 Hz组和10 Hz组(P< 0.01)。结论LIPEMF能明显促进ESC增殖．频率是影响ESC增殖的重要因素之一。Objective To observe the effects of a low intensity pulsed electromagnetic field （LIPEMF） on the differentiation and regulation of skin epidermal stem cells （ESC）, Methods Prepuces excised from healthy, young volunteers were used to culture ESC in vitro, identified by integrin β1 and keratin 19 （ K19 ）. The LIPEMF therapy group was divided into three sub-groups to receive 1 Hz, 10 Hz and 50 Hz fields, The ratio of clone cells formed was calculated. MTT was used to detect the growth curve of the cells. Results The LIPEMF stimulated the proliferation of ESC and the proliferation was different in every therapy group. The proliferation was highest in the 50 Hz group, who＇s growth curves were anterior to and higher than those of the control group. The integrin β1 and K19 dying results showed that ESC growth was positive and that the formation of ESC clone cells was faster in every therapy group than in the control group. Conclusion LIPEMF can stimulate the proliferation of ESC. The frequency is an important determinant of the proliferation rate,

关 键 词：表皮干细胞 脉冲电磁场 

分 类 号：R454[医药卫生—治疗学]